Asset Details
Do you wish to reserve the book?
Noninvasive, epigenetic age estimation in an elasmobranch, the cownose ray (Rhinoptera bonasus)
Hey, we have placed the reservation for you!
By the way, why not check out events that you can attend while you pick your title.
Oops! Something went wrong.
Looks like we were not able to place the reservation. Kindly try again later.
Are you sure you want to remove the book from the shelf?
Noninvasive, epigenetic age estimation in an elasmobranch, the cownose ray (Rhinoptera bonasus)
Do you wish to request the book?
Noninvasive, epigenetic age estimation in an elasmobranch, the cownose ray (Rhinoptera bonasus)
Please be aware that the book you have requested cannot be checked out. If you would like to checkout this book, you can reserve another copy
We have requested the book for you!
Your request is successful and it will be processed during the Library working hours. Please check the status of your request in My Requests.
Oops! Something went wrong.
Looks like we were not able to place your request. Kindly try again later.
Journal Article
Noninvasive, epigenetic age estimation in an elasmobranch, the cownose ray (Rhinoptera bonasus)
2024
Overview
Age data are essential for estimating life history parameters and are thus critical for population assessment, management, and conservation. Traditional vertebrae-based age estimation in elasmobranchs can be costly, time intensive, of low accuracy, and is by necessity lethal. Herein, epigenetic clocks were developed for an elasmobranch, the cownose ray (
Rhinoptera bonasus
), using aquarium-born individuals (
n
= 42) with known dates of birth (age range: 7−7,878 days or 0−21 years) and two tissue types (fin clips and whole blood) that can be sampled in a relatively non-invasive manner. Enzymatically-converted restriction site-associated DNA sequencing (ECrad-seq) was used to identify CpG sites that exhibited age-correlated DNA methylation. The epigenetic clocks developed were highly accurate (mean absolute error, MAE, < 0.75 years) and precise (
R
2
> 0.98). Age-associated CpG sites were identified across tissues, and a multi-tissue clock was also highly accurate (MAE < 1 year) and precise (
R
2
= 0.97). Using the developed fin clip clock, three wild-caught individuals of unknown age but managed in aquariums for > 22 years were predicted to be 22.10−23.49 years old. Overall, the results have important implications for future epigenetic clock development and noninvasive age estimation in elasmobranchs.
Publisher
Nature Publishing Group UK,Nature Publishing Group,Nature Portfolio
Subject
/ Age
/ Ageing
/ Animals
/ DNA
/ Humanities and Social Sciences
/ Rays
/ Science
/ Sequence Analysis, DNA - methods
/ Sharks
