Asset Details
Do you wish to reserve the book?
Laser-assisted microbial culturomics
Hey, we have placed the reservation for you!
By the way, why not check out events that you can attend while you pick your title.
Oops! Something went wrong.
Looks like we were not able to place the reservation. Kindly try again later.
Are you sure you want to remove the book from the shelf?
Laser-assisted microbial culturomics
Do you wish to request the book?
Laser-assisted microbial culturomics
Please be aware that the book you have requested cannot be checked out. If you would like to checkout this book, you can reserve another copy
We have requested the book for you!
Your request is successful and it will be processed during the Library working hours. Please check the status of your request in My Requests.
Oops! Something went wrong.
Looks like we were not able to place your request. Kindly try again later.
Journal Article
Laser-assisted microbial culturomics
2025
Overview
Even though metagenomics have revolutionized the characterization of the human microbiome, detailed mechanistic studies are impracticable, as there is a dearth of robust culture collections. We now describe the development and use of a laser-assisted culturomics platform, incorporating the elements of a bioprinter, the culture conditions, the methods to characterize the microorganisms and a biobank. With laser-assisted bioprinting, the microorganisms can be rapidly and precisely transferred from clinical biofilms to highly organized arrays of microbial colonies, which are suitable for co-culturing and molecular analyses. The presented technique has propagated 99 of 100 microbial species and recovered 79% of abundant species from dental plaque in accordance with full 16S rRNA gene profiling of 691,199 sequences. Microscopy, spectroscopy and enzyme assays have been used to guide isolations. Processing of oral biofilms from four individuals has yielded 249 representative isolates, from 14 classes and 124 species in total. Functional profiling with bioprinting has indicated commensals which could potentially contribute to disease development. Isolates from peri-implantitis cover 85.4% of the transcriptionally active clinical biofilms at genus level. Taken together, this work provides the basis for generating on-demand culture collections and biofilms for research and clinical use.
Representative microbial isolates and patient-specific biobanks are crucial for microbiome investigation and management. Here, authors develop laser-assisted microbial culturomics, combining high-throughput, precise bioprinting on diverse media with rapid, non-invasive analyses.
Publisher
Nature Publishing Group UK,Nature Publishing Group,Nature Portfolio
Subject
/ 45/22
/ 45/23
/ 49/47
/ Arrays
/ Bacteria - growth & development
/ Bacteria - isolation & purification
/ Biofilms
/ Biofilms - growth & development
/ Culture
/ Dental Plaque - microbiology
/ Humanities and Social Sciences
/ Humans
/ Lasers
/ RNA, Ribosomal, 16S - genetics
/ rRNA 16S
/ Science
/ Taxonomy
