Journal Article

Antimicrobial susceptibility profiles of Mycoplasma hyosynoviae strains isolated from five European countries between 2018 and 2023

Wehmann, Enikő, Catania, Salvatore, Bányai, Krisztián, Kreizinger, Zsuzsa, Nagy, Eszter Zsófia, Belecz, Nikolett, Gastaldelli, Michele, Földi, Dorottya, Költő, Karola, Merenda, Marianna, Depondt, Wouter, Vyt, Philip, Klein, Ulrich, Marton, Szilvia, Tóth, Lilla, Siesenop, Ute, Gyuranecz, Miklós, Spergser, Joachim

2025

Overview

Mycoplasma ( M .) hyosynoviae is a facultative pathogen, causing arthritis in finisher pigs world-wide. In the absence of a commercial vaccine improvement of housing conditions and antibiotic therapy are the only options to alleviate the clinical signs. This study aimed to determine antibiotic susceptibility profiles of 106 M . hyosynoviae isolates against ten antibiotics licensed for veterinary use in cases of arthritis. The isolates were collected between 2018 and 2023 from five European countries: Austria (n = 20), Belgium (n = 20), Germany (n = 25), Hungary (n = 21) and Italy (n = 20). The minimal inhibitory concentrations (MIC) were determined by broth micro-dilution assay. The tested isolates were highly susceptible to tiamulin (MIC 90  ≤ 0.039 µg/ml), tylvalosin (MIC 90  ≤ 0.039 µg/ml) and lincomycin (MIC 90  ≤ 0.25 µg/ml). Low concentrations of tylosin (MIC 90 0.5 µg/ml) and tilmicosin (MIC 90 1 µg/ml) inhibited the growth of the isolates. While moderate minimal inhibitory concentrations were detected for doxycycline (MIC 90 0.312 µg/ml), oxytetracycline (MIC 90 2 µg/ml), enrofloxacin (MIC 90 0.625 µg/ml) and florfenicol (MIC 90 2 µg/ml), only high concentrations of tulathromycin (MIC 90 64 µg/ml) inhibited the growth of the isolates. Statistical analysis revealed significant differences between countries in case of enrofloxacin, where the Hungarian isolates showed the lowest MIC values, and the German isolates the highest MIC values among the tested countries. Our results show that European M. hyosynoviae isolates are generally susceptible to the tested antibiotics with the exception of tulathromycin. The country specific differences indicate the importance of regular susceptibility testing of isolates on a Pan-European level.

• Publisher: Nature Publishing Group UK,Nature Publishing Group,Nature Portfolio
• Subject: 631/326/1320 / 631/326/22/1434 / Animals / Anti-Bacterial Agents - pharmacology / Antibiotics / Arthritis / Austria / Belgium / Broth micro-dilution / Diterpenes - pharmacology / Doxycycline / Doxycycline - pharmacology / Enrofloxacin / Europe / Florfenicol / Germany / Humanities and Social Sciences / Hungary / Italy / Lincomycin / Lincomycin - pharmacology / MIC / Microbial Sensitivity Tests / Minimum inhibitory concentration / multidisciplinary / Mycoplasma / Mycoplasma hyosynoviae / Mycoplasma hyosynoviae - drug effects / Mycoplasma Infections - drug therapy / Mycoplasma Infections - microbiology / Mycoplasma Infections - veterinary / Oxytetracycline / Science / Science (multidisciplinary) / Statistical analysis / Swine / Swine Diseases - microbiology / Tiamulin / Tilmicosin / Tylosin / Tylosin - analogs & derivatives / Tylosin - pharmacology