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Targeted Separation of COX-2 Inhibitor from Pterocephalus hookeri Using Preparative High-Performance Liquid Chromatography Directed by the Affinity Solid-Phase Extraction HPLC System
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Targeted Separation of COX-2 Inhibitor from Pterocephalus hookeri Using Preparative High-Performance Liquid Chromatography Directed by the Affinity Solid-Phase Extraction HPLC System
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Journal Article
Targeted Separation of COX-2 Inhibitor from Pterocephalus hookeri Using Preparative High-Performance Liquid Chromatography Directed by the Affinity Solid-Phase Extraction HPLC System
2021
Overview
Pterocephalus hookeri, as a kind of popular traditional Tibetan medicine, is reputed to treat inflammatory related diseases. In the present work, a cyclooxygenase-2 functionalized affinity solid-phase extraction HPLC system was developed and combined with preparative-HPLC for rapidly screening and separating cyclooxygenase-2 ligand from P. hookeri extracts. Firstly, ligands of cyclooxygenase-2 were screened from extracts by affinity solid-phase extraction HPLC system. Then directed by the screening results, the recognized potential active compounds were targeted separated. As a result, the major cyclooxygenase-2 inhibitor of P. hookeri was obtained with a purity of >95%, which was identified as sylvestroside I. To test the accuracy of this method, the anti-inflammatory activity of sylvestroside I was inspected in lipopolysaccharide-induced RAW 264.7 cells. The results show that sylvestroside I significantly suppressed the release of prostaglandin E2 with dose-dependent, which was in good agreement with the screening result of the affinity solid-phase method. This method of integration of screening and targeted separation proved to be very efficient for the recognition and isolation of cyclooxygenase-2 inhibitors from natural products.
Publisher
MDPI AG,MDPI
Subject
/ Animals
/ Anti-Inflammatory Agents - chemistry
/ Anti-Inflammatory Agents - metabolism
/ Anti-Inflammatory Agents - pharmacology
/ Cell Survival - drug effects
/ Chromatography, Affinity - methods
/ Chromatography, High Pressure Liquid - methods
/ Cyclooxygenase 2 - metabolism
/ Cyclooxygenase 2 Inhibitors - chemistry
/ Cyclooxygenase 2 Inhibitors - metabolism
/ Cyclooxygenase 2 Inhibitors - pharmacology
/ Enzymes
/ Ethanol
/ Ligands
/ Medicine, Tibetan Traditional - methods
/ Mice
/ Molecular Docking Simulation
/ Nonsteroidal anti-inflammatory drugs
