Journal Article

Himalayan Nettle Girardinia diversifolia as a Candidate Ingredient for Pharmaceutical and Nutraceutical Applications—Phytochemical Analysis and In Vitro Bioassays

Barbon Di Marco, Serena, Sharan Shrestha, Shyam, Biancorosso, Noemi, Maggi, Filippo, De Franco, Michele, Mahomoodally, Mohamad Fawzi, Rajbhandary, Sangeeta, Ferri, Nicola, Zengin, Gokhan, Pant, Deepak Raj, Dall Acqua, Stefano, Ferrarese, Irene, Sut, Stefania

2020

Overview

Girardinia diversifolia, also known as Himalayan nettle, is a perennial herb used in Nepal to make fiber as well as in traditional medicine for the treatment of several diseases. To date, phytochemical studies and biological assays on this plant are scarce. Thus, in the present work, the G. diversifolia extracts have been evaluated for their potential pharmaceutical, cosmetic and nutraceutical uses. For this purpose, detailed phytochemical analyses were performed, evidencing the presence of phytosterols, fatty acids, carotenoids, polyphenols and saponins. The most abundant secondary metabolites were β- and γ-sitosterol (11 and 9% dw, respectively), and trans syringin (0.5 mg/g) was the most abundant phenolic. Fatty acids with an abundant portion of unsaturated derivatives (linoleic and linolenic acid at 22.0 and 9.7 mg/g respectively), vitamin C (2.9 mg/g) and vitamin B2 (0.12 mg/g) were also present. The antioxidant activity was moderate while a significant ability to inhibit acetylcholinesterase (AChE), butyrilcholinesterase (BuChE), tyrosinase, α-amylase and α-glucosidase was observed. A cytotoxic effect was observed on human ovarian, pancreatic and hepatic cancer cell lines. The effect in hepatocarcinoma cells was associated to a downregulation of the low-density lipoprotein receptor (LDLR), a pivotal regulator of cellular cholesterol homeostasis. These data show the potential usefulness of this species for possible applications in pharmaceuticals, nutraceuticals and cosmetics.

• Publisher: MDPI AG,MDPI
• Subject: Anticholesteremic Agents - isolation & purification / Anticholesteremic Agents - pharmacology / Antioxidants - isolation & purification / Antioxidants - pharmacology / Ascorbic Acid - isolation & purification / Ascorbic Acid - pharmacology / Bioassays / Carotenoids / Carotenoids - isolation & purification / Carotenoids - pharmacology / Cell Line, Tumor / Cell Survival - drug effects / Chromatography / cytotoxicity / Cytotoxins - isolation & purification / Cytotoxins - pharmacology / Enzyme Inhibitors - isolation & purification / Enzyme Inhibitors - pharmacology / Enzymes / Fatty acids / Fatty Acids - isolation & purification / Fatty Acids - pharmacology / Flowers & plants / Functional foods & nutraceuticals / Glucosides - isolation & purification / Glucosides - pharmacology / Humans / Ions / LC-MS / LDLR / Leaves / Liver cancer / Mass spectrometry / Metabolites / NMR / Pharmaceuticals / Phenylpropionates - isolation & purification / Phenylpropionates - pharmacology / Phytochemicals / Phytochemicals - isolation & purification / Phytochemicals - pharmacology / phytosterols / Phytosterols - isolation & purification / Phytosterols - pharmacology / Plant Extracts - chemistry / polyphenols / Polyphenols - isolation & purification / Polyphenols - pharmacology / Receptors, LDL - antagonists & inhibitors / Receptors, LDL - genetics / Receptors, LDL - metabolism / Riboflavin - isolation & purification / Riboflavin - pharmacology / Saponins - isolation & purification / Saponins - pharmacology / Scientific imaging / Sensors / Sitosterols - isolation & purification / Sitosterols - pharmacology / Traditional medicine / Tuberculosis / Urticaceae - chemistry