Journal Article

The E3 ubiquitin ligase MARCH2 controls TNF-α mediated inflammation by autoubiquitination

Lee, Jong-Soo, Haluwana, D. K., Gayan Chathuranga, W. A., Choi, Youn Jung, Gamage, Nuwan, Weerawardhana, Asela, Kim, Tae-Hwan, Chathuranga, Kiramage, Subasinghe, Ashan, Rathnapala, Pramodya, Jung, Jae U., Seong, Yebin

2025

Overview

Background Regulation of the nuclear factor-kappa B (NF-kB) signaling pathway is a major host homeostatic mechanism for controlling hyper-inflammation or chronic inflammation. Despite extensive research, the regulatory factors of NF-kB signaling required to preserve homeostasis and control inflammatory disorders are not fully understood. Moreover, the role of MARCH2 in chronic inflammation models and the regulation of MARCH2 activation remain to be elucidated. Methods We monitored disease severity and mortality in MARCH2 −/− or MARCH2 +/+ mice induced experimental colitis. Susceptibility to DSS-induced experimental colitis was determined by various methods, including Swiss roll assay and fluorescein isothiocyanate (FITC)-dextran treatment, respectively. RNA-sequencing was conducted to recognize the inflammatory response-related genes in the distal colon of colitis-induced mice. Enzyme-linked immunosorbent assay (ELISA) was used to measure the cytokines and chemokines with in vitro and in vivo samples. Affinity purification and LC-MS/MS analysis were used to identify the MARCH2 interacting proteins and posttranslational modifications. The underlying mechanism was elucidated using immunoblotting, co-immunoprecipitation, ubiquitination assay, and confocal microscopy. Result Here, we report that MARCH2 −/− mice were more susceptible to experimental inflammatory bowel disease (IBD) due to the massive production of cytokines. Stimulation by inflammatory cytokines such as TNF induces dimerization of MARCH2 at a later stage and dimerized MARCH2 undergoes K63-linked autoubiquitination at lysine 127 and 238, which promotes NEMO recognition, ubiquitination and proteasomal degradation. We also show an interaction between MARCH2 and MARCH8 in resting cells that inhibits MARCH2 activation. Taken together, these findings provide new insights into the molecular mechanism of MARCH2 and suggest a crucial role of MARCH2 in the modulation of inflammation and cellular homeostasis. Conclusion Our results indicate that MARCH2 plays a critical role in regulating NEMO/IKKγ under the inflammatory and resting conditions, thereby suppressing excessive or unexpected inflammatory responses. Our findings here not only demonstrate a biological role of MARCH2 in inflammatory signaling pathways but also provide a novel insight in the underlying mechanism.

• Publisher: BioMed Central,Springer Nature B.V,BMC
• Subject: Animals / Autoubiquitination / Biomedical and Life Sciences / Cell activation / Cell Biology / Chemokines / Colitis / Colitis - chemically induced / Colitis - metabolism / Colitis - pathology / Colon / Confocal microscopy / Cytokines and Growth Factors / Dextran / Dimerization / Drinking water / Enzyme-linked immunosorbent assay / Fluorescein isothiocyanate / Homeostasis / Humans / IBD / Immunoblotting / Immunohistochemistry / Immunoprecipitation / Inflammation / Inflammation - metabolism / Inflammation - pathology / Inflammatory bowel disease / Inflammatory bowel diseases / Inflammatory diseases / Life Sciences / MARCH2 / MARCH8 / Mice / Mice, Inbred C57BL / Mice, Knockout / Molecular modelling / NF-κB protein / Oral administration / Permeability / Physiology / Proteasomes / Protein-Ligand Interactions / Proteins / Receptors / Signal transduction / Tumor Necrosis Factor-alpha - metabolism / Tumor necrosis factor-TNF / Tumor necrosis factor-α / Ubiquitin-protein ligase / Ubiquitin-Protein Ligases - deficiency / Ubiquitin-Protein Ligases - genetics / Ubiquitin-Protein Ligases - metabolism / Ubiquitination / Viral infections