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Kinetics of pIgR and IgM immune responses in snakehead (Channa argus) to inactivated Aeromonas hydrophila via immersion and intraperitoneal injection
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Kinetics of pIgR and IgM immune responses in snakehead (Channa argus) to inactivated Aeromonas hydrophila via immersion and intraperitoneal injection
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Journal Article
Kinetics of pIgR and IgM immune responses in snakehead (Channa argus) to inactivated Aeromonas hydrophila via immersion and intraperitoneal injection
2025
Overview
This research was intended to investigate the kinetics of polymeric immunoglobulin receptor (pIgR) and its ligand immunoglobulins (Ig) in snakehead (
Channa argus
) through different vaccine delivery methods. First, we employed Oxford Nanopore Technologies (ONT) sequencing technology to acquire the snakehead fish’s complete transcriptome, and the full coding sequence (CDS) of pIgR was identified and isolated through transcriptome sequencing named
Ch
pIgR. The CDS sequence spanned 1251 base pairs, translating into a protein that consists of 416 amino acids and has a molecular weight of around 45.80 kDa. The
Ch
pIgR had a pair of immunoglobulin-like domains in addition to a transmembrane region and an intracellular region. Furthermore, a polyclonal antibody targeting the snakehead recombinant
Ch
pIgR was constructed.
Ch
pIgR and IgM responses were analyzed after immunization with
Aeromonas hydrophila
. Although
Ch
pIgR
and
IgM
displayed a comparable transcription level,
Ch
pIgR
exhibited a more rapid increase and reached its peak earlier than
IgM
. Both vaccinated groups experienced an elevation in
ChpIgR
mRNA levels in the gill and spleen. On the other hand, the bath immersion group manifested that the skin, gills, and intestines had an elevated
IgM
mRNA expression, whereas the intraperitoneal vaccination group reported that the spleen and head kidney possessed a greater expression. The ELISA results indicated that the IgM and
Ch
pIgR levels reached their highest peak more rapidly in the skin and gill mucus in the bathing group, whereas they reached a greater peak in intestinal mucus in the injection group. The outcomes indicated that both bath and intraperitoneal vaccinations increased
Ch
pIgR and secretory Ig levels in mucus and bile, shedding light on the function of pIgR in immune response and laying the foundation for future studies on pIgR-linked immune defenses in teleosts.
Publisher
Nature Publishing Group UK,Nature Publishing Group,Nature Portfolio
Subject
/ Aeromonas hydrophila - immunology
/ Animals
/ Enzyme-linked immunosorbent assay
/ Fish Diseases - microbiology
/ Fish Diseases - prevention & control
/ Gills
/ Gram-Negative Bacterial Infections - immunology
/ Gram-Negative Bacterial Infections - prevention & control
/ Gram-Negative Bacterial Infections - veterinary
/ Humanities and Social Sciences
/ Immunoglobulin M - immunology
/ Kidneys
/ Kinetics
/ Mucus
/ Polymeric Immunoglobulin receptor (pIgR)
/ Receptors, Polymeric Immunoglobulin - genetics
/ Receptors, Polymeric Immunoglobulin - immunology
/ Receptors, Polymeric Immunoglobulin - metabolism
/ Science
/ Spleen
/ Vaccines
