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MINFLUX microscopy resolves subunits of the cardiac ryanodine receptor and its 3D orientation in cells
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MINFLUX microscopy resolves subunits of the cardiac ryanodine receptor and its 3D orientation in cells
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Journal Article
MINFLUX microscopy resolves subunits of the cardiac ryanodine receptor and its 3D orientation in cells
2025
Overview
The cardiac ryanodine receptor (RyR2) constitutes the molecular basis of the process of calcium-induced calcium release where activation of RyR2s can be locally regenerative. Here, we present purely optical data of RyR2 distribution with sub-molecular resolution by applying 3D MINFLUX microscopy. Using single-domain antibodies and DNA-PAINT we determine the location of individual RyR2 subunits with high precision (~3 nm) and resolve the 3D orientations of RyR2s in-situ. We measured labeling efficiencies of ~50%, implying RyR2 tetramer detection probability approaching 95%. In HEK293 cells, RyR2 expression was dense, with some clusters containing several hundred RyR2s. Ventricular myocytes from mice contained large clusters containing many tens of close-packed RyR2s, resolving apparent discrepancies between electron microscopy and previous super-resolution microscopy data. The methodology developed here reveals the full 3D morphological complexity of RyR2 channels and is applicable to other multi-subunit complexes in a variety of cell types.
Cardiac ryanodine receptors (RyR2) are critical for heart contraction. Here, the authors use 3D MINFLUX microscopy to image receptor subunits and RyR2 orientation with nanometre resolution, thereby providing a molecular view of the organisation and clustering of these cardiac muscle receptors.
Publisher
Nature Publishing Group UK,Nature Publishing Group,Nature Portfolio
Subject
/ 14/63
/ Animals
/ Calcium
/ Heart Ventricles - metabolism
/ Humanities and Social Sciences
/ Humans
/ Imaging, Three-Dimensional - methods
/ Labeling
/ Mice
/ Myocytes
/ Myocytes, Cardiac - metabolism
/ Protein Subunits - metabolism
/ Ryanodine Receptor Calcium Release Channel - chemistry
/ Ryanodine Receptor Calcium Release Channel - genetics
/ Ryanodine Receptor Calcium Release Channel - metabolism
/ Ryanodine Receptor Calcium Release Channel - ultrastructure
/ Science
