Journal Article

Cryo-EM structures of human ClpXP reveal mechanisms of assembly and proteolytic activation

Chen, Wenqian, Yang, Jie, Lander, Gabriel C.

2026

Overview

The human ClpXP complex (hClpXP) orchestrates mitochondrial protein quality control through targeted degradation of misfolded and unnecessary proteins. While bacterial ClpXP systems are well characterized, the assembly and regulation of human ClpXP remain poorly understood. In this study, we elucidate the complete assembly pathway of hClpXP through high-resolution cryo-electron microscopy (cryo-EM) structures. Our findings confirm that hClpP exists as a single-ring heptamer in isolation and reveal a previously undocumented initial assembly complex in which hexameric hClpX first engages with heptameric hClpP. We further demonstrate how this interaction drives substantial conformational rearrangements that facilitate the formation of tetradecameric hClpP within the fully assembled complex. Notably, we characterize a unique eukaryotic sequence in hClpX, termed the E-loop, which plays a critical role in stabilizing hexamer assembly and maintaining ATPase activity. Additionally, we show that peptide binding at the hClpP active site triggers further structural changes essential for achieving full proteolytic competence. Together, these structures provide unprecedented mechanistic insights into the stepwise assembly and activation of hClpXP, significantly advancing our understanding of this essential mitochondrial protein degradation machinery. Mitochondrial ClpXP maintains protein quality through targeted degradation. Here, the authors use cryo-EM to define the stepwise assembly of human ClpXP, identifying key intermediates and a unique E-loop element that regulates complex formation and proteolytic activation.

• Publisher: Nature Publishing Group UK,Nature Publishing Group,Nature Portfolio
• Subject: 101/28 / 631/337/470 / 631/45/535/1258/1259 / 82/16 / 82/80 / 82/83 / Biodegradation / Catalytic Domain / Complex formation / Cryoelectron Microscopy / Degradation / Electron microscopy / Endopeptidase Clp - chemistry / Endopeptidase Clp - genetics / Endopeptidase Clp - metabolism / Endopeptidase Clp - ultrastructure / Homeostasis / Humanities and Social Sciences / Humans / Intermediates / Mitochondria / Mitochondrial DNA / Models, Molecular / Molecular Chaperones - chemistry / Molecular Chaperones - genetics / Molecular Chaperones - metabolism / Molecular Chaperones - ultrastructure / multidisciplinary / Optimization / Peptides / Proteases / Protein Binding / Protein Conformation / Protein folding / Protein Multimerization / Proteins / Proteolysis / Quality control / Science / Science (multidisciplinary)