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Improving tolerance to fluctuating light through adaptive laboratory evolution in the cyanobacterium Synechocystis
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Improving tolerance to fluctuating light through adaptive laboratory evolution in the cyanobacterium Synechocystis
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Journal Article
Improving tolerance to fluctuating light through adaptive laboratory evolution in the cyanobacterium Synechocystis
2026
Overview
Fluctuating light (FL) poses a challenge to cyanobacteria by disrupting photosynthesis and damaging photosystems. Although key FL tolerance components are known, their genetic enhancement remains unexplored. We evolve
Synechocystis
PCC 6803 under two FL regimes (one lethal to the starter strain, LT) in order to identify previously unknown adaptive alleles. Our analysis reveals 44 mutations, 28 of which impact proteins/RNAs. Mutations in Pam68 (PSII assembly) and Sll0518, present in all strains, enhance non-lethal FL tolerance in LT. Mutated Pam68 increased PSII abundance and activity. A gain-of-function mutation in RpaB (regulator of phycobilisome association B) significantly increases tolerance to both lethal FL and high-light conditions. This is associated with an increased PSI/PSII ratio and downregulation of light harvesting. In summary, our results suggest that adaptive laboratory evolution can simultaneously identify FL tolerance factors and their advantageous alleles. The identified point mutations rewire multiple protective responses by as yet unknown molecular mechanisms.
Adaptive laboratory evolution enables cyanobacteria to tolerate fluctuating light by acquiring beneficial mutations. Key mutations improve photosystem performance or reduce light harvesting, enhancing survival under stressful light conditions.
Publisher
Nature Publishing Group UK,Nature Publishing Group,Nature Portfolio
Subject
/ 45/41
/ 82/1
/ 82/29
/ Adaptation, Physiological - genetics
/ Alleles
/ Bacterial Proteins - genetics
/ Bacterial Proteins - metabolism
/ Cloning
/ Directed Molecular Evolution
/ Genomes
/ Humanities and Social Sciences
/ Light
/ Mutation
/ Photosynthesis - radiation effects
/ Photosystem II Protein Complex - genetics
/ Photosystem II Protein Complex - metabolism
/ Protocol
/ Science
