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A transpupillary approach for crosslinking Guinea pig sclera using WST11 and near-infrared light
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A transpupillary approach for crosslinking Guinea pig sclera using WST11 and near-infrared light
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Journal Article
A transpupillary approach for crosslinking Guinea pig sclera using WST11 and near-infrared light
2026
Overview
Crosslinking strengthens the sclera and holds potential as a treatment for myopia. This study aims to identify optimal crosslinking parameters in guinea pigs using WST11 with dextran followed by near-infrared (NIR) illumination. Guinea pig eyes were incubated in WST11 with 2, 5 or 10% dextran, and penetration depth was assessed by fluorescence microscopy. Crosslinking efficacy was measured as thermal stability using a thermal degradation assay, following incubation in WST11 + 10% dextran (WST-D) for 30 min and NIR irradiation at 10 mW/cm
2
or 20 mW/cm
2
for 10, 20 and 30 min. The optimized parameters were then applied in vivo in 6-month-old guinea pigs.
Ex vivo
treatment using the optimal crosslinking parameters (WST-D, 30 min; NIR, 10 mW/cm
2
, 30 min) resulted in the highest thermal degradation midpoint (
ΔT
50
: 6.8), significantly higher than untreated controls (
p
= 0.0006), with WST-D penetration limited to the sclera. Efficacy was greater in eyes obtained from older compared to younger guinea pigs (
p
= 0.02).
In vivo
, WST-D/NIR treatment resulted in significant crosslinking compared to untreated controls (equatorial,
ΔT
50
: 3.7,
p
< 0.0001; posterior,
ΔT
50
: 3.4,
p
= 0.01). WST-D/NIR treatment effectively induces scleral crosslinking, with age-related differences suggesting the need for personalized treatment.
Publisher
Nature Publishing Group UK,Nature Publishing Group,Nature Portfolio
Subject
