Journal Article

Methods for data analysis of resting energy expenditure measured using indirect calorimetry

Gonçalves, Ezequiel M., Borges, Juliano H., Guerra-Júnior, Gil

2019

Overview

•Reduced steady-state methods during 10 min of measurement overestimate the resting energy expenditure (REE).•Interval methods during 10 and 30 min of measurement overestimate the REE.•We recommend 5 min in steady state during 30 min of measurement to estimate the REE. The aim of this study was to test the accuracy of different methods of resting energy expenditure (REE) data analysis using indirect calorimetry (IC) during traditional (30 min) and abbreviated (10 min) protocols. Fifteen women and 15 men (21–34 y of age) completed two consecutive 30-min IC measurements. Body composition was measured using dual-energy x-ray absorptiometry. The reference method for REE analysis was 5 min in steady state (SS) during 30 min (first 5 min discarded). REE measurements were randomized to define a reference or testing method. An interval method was defined using 25, 20, and 15 min (with first 5, 10, and 15 min discarded, respectively), during 30 min, and 5 min (first 5 min discarded) during 10-min intervals. The SS method was defined using 5 min in SS (first 5 min discarded) during 30 min, 5, 4, and 3 min in SS during 10-min (first 5 min discarded) intervals. Interval methods during 30 min and SS and interval methods during 10 min demonstrated large bias with significantly high REEs compared to the reference method (78.8–109.0 kcal/d, all P < 0.001). Testing methods demonstrated large upper limits of agreement between 225.2 and 322.8 kcal/d. No mean differences (P > 0.05), small bias (14.3 kcal/d), and narrow limits of agreement (–125.8 to 154.4 kcal/d) were observed between 5-min SS during 30 min and the reference method. All interval methods and SS methods during 10 min overestimated REE. We recommend using 5-min SS during 30 min. The measurement may be repeated until all participants achieve SS.

• Publisher: Elsevier Inc,Elsevier Limited
• Subject: Absorptiometry, Photon - methods / Absorptiometry, Photon - statistics & numerical data / Adult / Basal Metabolism / Bias / Body Composition / Calorimetry / Calorimetry, Indirect - methods / Carbon dioxide / Data analysis / Data Interpretation, Statistical / Data processing / Dual energy X-ray absorptiometry / Energy / Energy expenditure / Energy measurement / Energy Metabolism / Energy requirements / Female / Heat measurement / Humans / Intervals / Male / men / Metabolic cart / Metabolism / Methods / Reference Values / Reproducibility of Results / Rest - physiology / resting energy expenditure / Resting metabolic rate / Steady state / Studies / Test procedures / Time Factors / women / Young Adult