Journal Article

Characterization of early myocardial inflammation in ischemia-reperfusion injury

Liu, Hanrui, Wu, Qihong, Yang, Mengxi, Sun, Ran, Guo, Yingkun, Xu, Rong, Zhang, Kun, Li, Kuan, Xue, Yiyuan, Xu, Huayan

2023

Overview

Myocardial injury may be caused by myocardial ischemia-reperfusion (IR), and salvaging such an injury is still a great challenge in clinical practice. This study comprehensively characterized the physiopathologic changes of myocardial injury after IR to explore the underlying mechanism in the early reperfusion phase with particular emphasis on early myocardial inflammation. The experimental IR model was obtained by the left anterior descending artery's transient ligation of C57BL/6 mice. T2W signals of all mice showed increased signal at different IR stages. It was positively correlated with inflammatory cytokines and cells. T2W imaging by 7.0 T MRI surprisingly detected signal enhancement, but histopathology and flow cytometry did not reveal any inflammatory cells infiltration within 3 h after IR. Cardiomyocyte swelling and increased vascular permeability were observed by WGA staining and ultrastructural analysis, respectively. The 3 h IR group showed that the cardiomyocytes were severely affected with disintegrating myofilaments and mitochondria. Both VEGF and phosphorylated Src protein were markedly expressed in the 3 h IR group in comparison with the sham group, and TUNEL staining displayed little positive cells. Cleaved caspase-3 apoptin also has similar expression levels with that of the sham group. Resident macrophages had notably become M1 phenotype. The T2W signal was still elevated, and we observed that collagen deposition occurred from 1 to 7 days. The inflammation response during the first week after reperfusion injury gradually increase 3 h later, but the main manifestation before that was edema. This study indicated that the first 3 h may be crucial to the early rescue process for reperfusion-induced myocardial injury due to inflammatory cell infiltration absence and apoptosis.

• Publisher: Frontiers Media SA,Frontiers Media S.A
• Subject: Animals / Antibodies / Apoptosis / Cardiomyocytes / Caspase-3 / Edema / Flow cytometry / Heart attacks / Immunology / Infiltration / Inflammation / Inflammation - metabolism / Ischemia / Laboratory animals / Macrophages / Magnetic resonance imaging / Mice / Mice, Inbred C57BL / MRI / Myocardial ischemia / myocardial ischemia reperfusion injury / Myocardial Reperfusion Injury - metabolism / Myocardium - pathology / Myocytes, Cardiac - metabolism / Ostomy / Phenotypes / Reperfusion / Src protein / Vascular endothelial growth factor