Asset Details
Do you wish to reserve the book?
The effect of Matrigel as scaffold material for neural stem cell transplantation for treating spinal cord injury
Hey, we have placed the reservation for you!
By the way, why not check out events that you can attend while you pick your title.
Oops! Something went wrong.
Looks like we were not able to place the reservation. Kindly try again later.
Are you sure you want to remove the book from the shelf?
The effect of Matrigel as scaffold material for neural stem cell transplantation for treating spinal cord injury
Do you wish to request the book?
The effect of Matrigel as scaffold material for neural stem cell transplantation for treating spinal cord injury
Please be aware that the book you have requested cannot be checked out. If you would like to checkout this book, you can reserve another copy
We have requested the book for you!
Your request is successful and it will be processed during the Library working hours. Please check the status of your request in My Requests.
Oops! Something went wrong.
Looks like we were not able to place your request. Kindly try again later.
Journal Article
The effect of Matrigel as scaffold material for neural stem cell transplantation for treating spinal cord injury
2020
Overview
Traumatic injury to the spinal cord causes permanent loss of function and major personal, social, and economic problems. Cell-based delivery strategies is a promising approach for treating spinal cord injury (SCI). However, the inhospitable microenvironment in the injured spinal cord results in poor cell survival and uncontrolled differentiation of the transplanted stem cells. The combination of a scaffold with cells has been developed with a tendency for achieving greater survival and integration with the host tissue. We investigated the effect of Matrigel combined with neural stem cells (NSCs)
in vitro
and
in vivo
. We compared the effect of different types of scaffold on the survival and differentiation of brain-derived NSCs in an
in vitro
culture. Subsequently, NSCs were transplanted subcutaneously into nude mice to detect graft survival and differentiation
in vivo
. Finally, phosphate-buffered saline (PBS), Matrigel alone, or Matrigel seeded with NSCs was injected into 48 subacute, clinically relevant rat models of SCI (16 rats per group). Matrigel supported cell survival and differentiation efficiently
in vitro
and
in vivo
. SCI rats transplanted with NSCs in Matrigel showed improved behavioral recovery and neuronal and reactive astrocyte marker expression levels compared to PBS- or Matrigel-transplanted rats. Functional repair and neuronal and reactive astrocyte marker expression was slightly improved in the Matrigel-alone group relative to the PBS group, but not statistically significantly. These data suggest that Matrigel is a promising scaffold material for cell transplantation to the injured spinal cord.
Publisher
Nature Publishing Group UK,Nature Publishing Group
Subject
/ 13/51
/ 14/105
/ 14/63
/ 59
/ 82
/ 82/1
/ Animals
/ Cell Differentiation - drug effects
/ Cell Survival - drug effects
/ Gene Expression Regulation - drug effects
/ Humanities and Social Sciences
/ Humans
/ Neural Stem Cells - drug effects
/ Neural Stem Cells - transplantation
/ Proteoglycans - pharmacology
/ Rats
/ Science
/ Spinal Cord Injuries - physiopathology
/ Spinal Cord Injuries - therapy
/ Survival
