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Enzyme-Microbe Synergy During Cellulose Hydrolysis by Clostridium thermocellum
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Enzyme-Microbe Synergy During Cellulose Hydrolysis by Clostridium thermocellum
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Journal Article
Enzyme-Microbe Synergy During Cellulose Hydrolysis by Clostridium thermocellum
2006
Overview
Specific cellulose hydrolysis rates (g of cellulose/g of cellulase per h) were shown to be substantially higher (2.7- to 4.7-fold) for growing cultures of Clostridium thermocellum as compared with purified cellulase preparations from this organism in controlled experiments involving both batch and continuous cultures. This \"enzyme-microbe synergy\" requires the presence of metabolically active cellulolytic microbes, is not explained by removal of hydrolysis products from the bulk fermentation broth, and appears due to surface phenomena involving adherent cellulolytic microorganisms. Results support the desirability of biotechnological processes featuring microbial conversion of cellulosic biomass to ethanol (or other products) in the absence of added saccharolytic enzymes.
Publisher
National Academy of Sciences,National Acad Sciences
