Journal Article

Myo-inositol affects the health, metabolome, and lactation performance of peripartum dairy cows

Zhao, Wei, Aschalew, Natnael D., Zhen, Yu-guo, Sun, Wu-wen, Zhao, Zhi-kun, Wang, Tao, Dong, Jia-nan, Qu, Ke, Lin, Zi-chen, Ta, Na, Zhao, Yang, Qin, Gui-xin, Liu, Yong-jun, Sun, Zhe, Zhang, Wei-gang, Wang, Chun-zheng

2025

Overview

Yeast culture (YC) has become a crucial microecological preparation for regulating the ruminal environment and improving ruminant health. -inositol is an effective substance in YC, our study aimed to investigate whether -inositol can regulate metabolism and influence the health of dairy cows. Therefore, this study explored the effects of -inositol on the health, metabolome, and lactation performance of peripartum dairy cows using both and models. The ANKOM RFS gas production system was used for the experiment; Twenty four healthy Holstein cows were assigned to CON group (n = 4; no supplementation of YC or -inositol), YC group (n = 10; 2 g/kg YC), and IN65 group (n = 10; 2 g/kg YC + 16 mg/kg -inositol) for experiment. The concentrations of five volatile fatty acids (acetic acid, butyric acid, isobutyric acid, valeric acid, and isovaleric acid), and total volatile fatty acids were significantly higher in the IN65 group compared to the CON group at 6 h ( < 0.01). Therefore, 2 g/kg YC plus 16 mg/kg -inositol was selected for experiment. It was found that the average milk production of the IN65 group increased by 4.00% and 4.05% respectively, compared to the control group and YC group on day 21 postpartum. The levels of superoxide dismutase (SOD), total antioxidant status (TAS), and total oxidative status (TOS) were significantly higher on day 21 compared to day 1 postpartum ( < 0.01). However, a trend indicating a decrease in immune function was observed in the IN65 group compared to the CON group. These findings suggested that -inositol may improve the health of postpartum dairy cows. Additionally, Mantel analysis showed acetic acid was positively correlated with D-glyceric acid and xanthosine. Similarly, D-glyceric acid, inosine 5-monophosphate, and xanthosine were positively correlated with microbiota at the genus, such as and . In summary, -inositol influences energy metabolism by modulating rumen microbial abundance, improving dairy cow health, and increasing milk production.

• Publisher: Frontiers Media SA,Frontiers Media S.A
• Subject: Acetic acid / Animal Feed / Animal lactation / Animals / Butyric acid / Cattle / Cow's milk / Dairy cattle / Dietary Supplements / Energy metabolism / Experiments / Fatty Acids, Volatile - metabolism / Feeds / Female / Fermentation / Gas production / health / Immune response / Immunology / Inositol / Inositol - administration & dosage / Inositol - pharmacology / Lactation / Lactation - drug effects / Metabolism / Metabolome - drug effects / metabolomes / Microbiota / Milk - metabolism / Milk production / myo-inositol / Nitrogen / Oxidation / peripartum dairy cows / Peripartum Period / Postpartum / Postpartum period / Pregnancy / Probiotics / Protozoa / Superoxide dismutase / Valeric acid / Volatile fatty acids