Journal Article

Disseminated melanized fungal infection due to Cladosporium halotolerans in a dog coinfected with canine adenovirus-1 and canine parvovirus-2

Pretto-Giordano, Lucienne Garcia, de Mello Zanim Michelazzo, Mariana, Viana, Nayara Emily, Vilas-Boas, Laurival Antonio, Headley, Selwyn Arlington, Pereira, Yuri Lima, da Costa Flaiban, Karina Keller Marques, Gomes, Lucas Alécio, Elias, Bruno, da Silva, Jhonata Fragoso, da Silva, Felipe Eduardo Scardovelli, Alfieri, Amauri Alcindo

2019

Overview

This report presents the pathologic findings associated with disseminated infection due to Cladosporium halotolerans in a dog that was simultaneously infected with canine adenovirus-1 (CAdV-1) and canine parvovirus-2 (CPV-2). A 12-year-old, mixed breed dog, with a clinical history of neurological manifestations was submitted for routine autopsy due to poor prognosis. The principal pathologic findings were mycotic necrotizing nephritis, hepatitis, and splenitis with embolic dissemination to the brain resulting in mycotic necrotizing meningoencephalitis, ventriculitis, choroid plexitis, and obstructive hydrocephalus associated with intralesional and intravascular septate pigmented fungi. PCR and sequencing of the ITS region of fungi revealed that the intralesional fungal organisms had 82% nucleotide identity with members of the Cladosporium sphaerospermum complex of organisms. However, a PCR assay and sequencing of the beta tubulin gene confirmed that the organism identified in this dog had 100% nucleotide sequence identity with C. halotolerans . Using immunohistochemistry, intralesional antigens of CAdV-1 were identified within the epithelial cells of the liver and lungs; there was positive immunolabeling for CPV-2 antigens in degenerated cardiomyocytes. These findings confirmed the active participation of C. halotolerans in the development of disseminated cladosporiosis in this dog and represent a rare occurrence of concomitant infection with CAdV-1 and CPV-2.

• Publisher: Springer International Publishing,Springer Nature B.V
• Subject: Adenoviridae Infections - veterinary / Adenoviridae Infections - virology / Adenoviruses / Adenoviruses, Canine - classification / Adenoviruses, Canine - genetics / Adenoviruses, Canine - isolation & purification / Animals / Antigens / Autopsies / Autopsy / Biomedical and Life Sciences / Cardiomyocytes / Cladosporium / Cladosporium - classification / Cladosporium - genetics / Cladosporium - isolation & purification / Cladosporium halotolerans / Coinfection - microbiology / Coinfection - veterinary / Coinfection - virology / Disseminated infection / Dog Diseases - microbiology / Dog Diseases - virology / Dogs / Epithelial cells / Epithelium / Food Microbiology / Fungi / Gene sequencing / Hepatitis / Hepatocytes / Hydrocephalus / Immunohistochemistry / Infections / Infectious diseases / Kidneys / Life Sciences / Liver / Lungs / Medical Microbiology / Melanins - metabolism / Meningoencephalitis / Microbial Ecology / Microbial Genetics and Genomics / Microbiology / Mycology / Mycoses - microbiology / Mycoses - veterinary / Nephritis / Nucleotide sequence / Nucleotides / Organisms / Parvoviridae Infections - veterinary / Parvoviridae Infections - virology / Parvovirus, Canine - classification / Parvovirus, Canine - genetics / Parvovirus, Canine - isolation & purification / Parvoviruses / Pathogens / Polymerase chain reaction / Protozoa / Serology / Small intestine / Spleen / Thrombosis / Tubulin / Ventriculitis / Veterinary medicine / Veterinary Microbiology - Research Paper