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Affinity Tag-Free Purification of SARS-CoV-2 N Protein and Its Crystal Structure in Complex with ssDNA
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Affinity Tag-Free Purification of SARS-CoV-2 N Protein and Its Crystal Structure in Complex with ssDNA
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Journal Article
Affinity Tag-Free Purification of SARS-CoV-2 N Protein and Its Crystal Structure in Complex with ssDNA
2024
Overview
The nucleocapsid (N) protein is one of the four structural proteins in SARS-CoV-2, playing key roles in viral assembly, immune evasion, and stability. One of its primary functions is to protect viral RNA by forming the nucleocapsid. However, the precise mechanisms by which the N protein interacts with viral RNA and assembles into a nucleocapsid remain unclear. Compared to other SARS-CoV-2 components, targeting the N protein has several advantages: it exhibits higher sequence conservation, lower mutation rates, and stronger immunogenicity, making it an attractive target for antiviral drug development and diagnostics. Therefore, a detailed understanding of the N protein’s structure is essential for deciphering its role in viral assembly and developing effective therapeutics. In this study, we report the expression and purification of a soluble recombinant N protein, along with a 1.55 Å resolution crystal structure of its nucleic acid-binding domain (N-NTD) in complex with ssDNA. Our structure revealed new insights into the conformation and interaction of the flexible N-arm, which could aid in understanding nucleocapsid assembly. Additionally, we identified residues that are critical for ssDNA interaction.
Publisher
MDPI AG,MDPI
Subject
/ Codes
/ Coronavirus Nucleocapsid Proteins - chemistry
/ Coronavirus Nucleocapsid Proteins - genetics
/ Coronavirus Nucleocapsid Proteins - isolation & purification
/ Coronavirus Nucleocapsid Proteins - metabolism
/ COVID-19
/ Crystals
/ DNA, Single-Stranded - chemistry
/ DNA, Single-Stranded - metabolism
/ E coli
/ Genomes
/ Humans
/ Nucleocapsid Proteins - chemistry
/ Nucleocapsid Proteins - genetics
/ Nucleocapsid Proteins - isolation & purification
/ Nucleocapsid Proteins - metabolism
/ Phosphoproteins - isolation & purification
/ Phosphoproteins - metabolism
/ Proteins
/ Recombinant Proteins - chemistry
/ Recombinant Proteins - genetics
/ Recombinant Proteins - isolation & purification
/ Recombinant Proteins - metabolism
/ RNA
