Journal Article

Anti-Inflammatory Effects of Melatonin and 5-Methoxytryptophol on Lipopolysaccharide-Induced Acute Pulpitis in Rats

Aksoy, Umut, Sebai, Abdullah, Savtekin, Gökçe, Özkayalar, Hanife, Sayıner, Serkan, Kermeoğlu, Fatma, Şehirli, Ahmet Özer

2021

Overview

Aim. The aim of this study was to investigate the possible therapeutic impacts of two pineal hormones, melatonin and 5-methoxytryptophol (5-MTX), in a rat model of acute pulpitis by analyzing biochemical and histopathological parameters. Methods. This research was done using 32 male and female Wistar albino rats with weight between 200 and 250 g. The rats were randomly divided into four groups: a control group (rats without any treatment), acute pulpitis (AP) group, AP+melatonin group, and AP+5-MTX group. In the AP-induced groups, the crowns of the upper left incisors were removed horizontally. Lipopolysaccharide solution was applied to the exposed pulp tissue before the canal orifices were sealed with a temporary filling material. Melatonin (10 mg/kg) and 5-MTX (5 mg/kg) were administered intraperitoneally. The rats were sacrificed 24 hours after pulp injury, and trunk blood and pulp samples were collected. The concentrations of TNF-α, IL-1β, MMP-1, and MMP-2 in sera and pulp samples were determined using ELISA assay kits. Results. TNF-α, IL-1β, MMP-1, and MMP-2 levels in the serum and pulp tissues were considerably higher in the AP group than the control group (p<0.01‐0.001). In the AP+melatonin and AP+5-MTX groups, TNF-α, IL-1β, MMP-1, and MMP-2 levels in the serum and pulp tissues were significantly lower than in the AP group (p<0.05‐0.001). Conclusions. Both melatonin and 5-MTX provided protective effects on acute pulpitis, which indicates they may be promising as a therapeutic strategy for oral disease.

• Publisher: Hindawi,John Wiley & Sons, Inc
• Subject: Abscesses / Acute Disease / Animal models / Animals / Anti-Inflammatory Agents - pharmacology / Anti-inflammatory drugs / Care and treatment / Cell adhesion & migration / Cytokines / Diagnosis / Dosage and administration / Enzyme-linked immunosorbent assay / Female / Gelatinase A / Health aspects / Hormones / IL-1β / Incisors / Indoles - pharmacology / Inflammation / Interleukin-1beta - blood / Interstitial collagenase / Laboratories / Lipopolysaccharides / Lipopolysaccharides - toxicity / Male / Matrix metalloproteinase / Matrix Metalloproteinase 1 - blood / Matrix Metalloproteinase 2 - blood / Medical research / Medicine, Experimental / Melatonin / Melatonin - pharmacology / Methods / Oral diseases / Orifices / Pineal gland / Proteins / Pulpitis / Pulpitis - blood / Pulpitis - chemically induced / Pulpitis - drug therapy / Pulpitis - pathology / Rats / Rats, Wistar / Risk factors / Teeth / Tissues / Tumor Necrosis Factor-alpha - blood / Tumor necrosis factor-TNF / Tumor necrosis factor-α