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Immunofluorescence identifies distinct subsets of endothelial cells in the human liver
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Immunofluorescence identifies distinct subsets of endothelial cells in the human liver
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Journal Article
Immunofluorescence identifies distinct subsets of endothelial cells in the human liver
2017
Overview
As well as systemic vascular endothelial cells, the liver has specialised sinusoidal endothelial cells (LSEC). LSEC dysfunction has been documented in many diseased states yet their phenotype in normal human liver has not been comprehensively assessed. Our aim was to improve characterisation of subsets of endothelial cells and associated pericytes in the human liver. Immunofluorescence microscopy was performed on normal human liver tissue samples to assess endothelial and structural proteins in a minimum of three donors. LSEC are distributed in an acinar pattern and universally express CD36, but two distinctive subsets of LSEC can be identified in different acinar zones. Type 1 LSEC are CD36
hi
CD32
−
CD14
−
LYVE-1
−
and are located in acinar zone 1 of the lobule, while Type 2 LSEC are LYVE-1
+
CD32
hi
CD14
+
CD54
+
CD36
mid-lo
and are located in acinar zones 2 and 3 of the lobule. Portal tracts and central veins can be identified using markers for systemic vascular endothelia and pericytes, none of which are expressed by LSEC. In areas of low hydrostatic pressure LSEC are lined by stellate cells that express the pericyte marker CD146. Our findings identify distinctive populations of LSEC and distinguish these cells from adjacent stellate cells, systemic vasculature and pericytes in different zones of the liver acinus.
Publisher
Nature Publishing Group UK,Nature Publishing Group
Subject
/ 14/1
/ 14/34
/ 14/35
/ 14/63
/ 692/4017
/ 692/53
/ Adult
/ Endothelial Cells - cytology
/ Endothelial Cells - metabolism
/ Endothelium, Vascular - cytology
/ Endothelium, Vascular - metabolism
/ Female
/ Hepatic Stellate Cells - cytology
/ Hepatic Stellate Cells - metabolism
/ Humanities and Social Sciences
/ Humans
/ Lipopolysaccharide Receptors - metabolism
/ Liver
/ Male
/ Microscopy, Fluorescence - methods
/ Science
/ Veins
