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Paraspeckles modulate the intranuclear distribution of paraspeckle-associated Ctn RNA
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Paraspeckles modulate the intranuclear distribution of paraspeckle-associated Ctn RNA
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Journal Article
Paraspeckles modulate the intranuclear distribution of paraspeckle-associated Ctn RNA
2016
Overview
Paraspeckles are sub-nuclear domains that are nucleated by long noncoding RNA
Neat1
. While interaction of protein components of paraspeckles and
Neat1
is understood, there is limited information on the interaction of non-structural RNA components with paraspeckles. Here, by varying paraspeckle number and size, we investigate how paraspeckles influence the nuclear organization of their non-structural RNA component
Ctn RNA
. Our results show that
Ctn RNA
remains nuclear-retained in the absence of intact paraspeckles, suggesting that they do not regulate nuclear retention of
Ctn RNA
. In the absence of
Neat1
,
Ctn RNA
continues to interact with paraspeckle protein NonO to form residual nuclear foci. In addition, in the absence of
Neat1
-nucleated paraspeckles, a subset of
Ctn RNA
localizes to the perinucleolar regions. Concomitant with increase in number of paraspeckles, transcriptional reactivation resulted in increased number of paraspeckle-localized
Ctn RNA
foci. Similar to
Neat1
, proteasome inhibition altered the localization of
Ctn RNA
, where it formed enlarged paraspeckle-like foci. Super-resolution structured illumination microscopic analyses revealed that in paraspeckles,
Ctn RNA
partially co-localized with
Neat1
, and displayed a more heterogeneous intra-paraspeckle localization. Collectively, these results show that while paraspeckles do not influence nuclear retention of
Ctn RNA
, they modulate its intranuclear compartmentalization.
Publisher
Nature Publishing Group UK,Nature Publishing Group
