Journal Article

A novel cell-cycle-indicator, mVenus-p27K−, identifies quiescent cells and visualizes G0–G1 transition

Maehara, Akie, Kitamura, Toshio, Sakaue-Sawano, Asako, Miyawaki, Atsushi, Nishimura, Koutarou, Kitaura, Jiro, Niida, Atsushi, Miyano, Satoru, Kiyonari, Hiroshi, Togami, Katsuhiro, Izawa, Kumi, Aburatani, Hiroyuki, Oki, Toshihiko

2014

Overview

The quiescent (G0) phase of the cell cycle is the reversible phase from which the cells exit from the cell cycle. Due to the difficulty of defining the G0 phase, quiescent cells have not been well characterized. In this study, a fusion protein consisting of mVenus and a defective mutant of CDK inhibitor, p27 (p27K − ) was shown to be able to identify and isolate a population of quiescent cells and to effectively visualize the G0 to G1 transition. By comparing the expression profiles of the G0 and G1 cells defined by mVenus-p27K − , we have identified molecular features of quiescent cells. Quiescence is also an important feature of many types of stem cells and mVenus-p27K − -transgenic mice enabled the detection of the quiescent cells with muscle stem cell markers in muscle in vivo. The mVenus-p27K − probe could be useful in investigating stem cells as well as quiescent cells.

• Publisher: Nature Publishing Group UK,Nature Publishing Group
• Subject: 14 / 14/35 / 3T3 Cells / 631/1647/245/2225 / 631/80/641/2350 / Animals / Apoptosis Regulatory Proteins - biosynthesis / Apoptosis Regulatory Proteins - genetics / Bacterial Proteins - genetics / Carrier Proteins - biosynthesis / Carrier Proteins - genetics / Cell cycle / Cell Line / Cyclin-dependent kinase / Cyclin-Dependent Kinase Inhibitor p16 - biosynthesis / Cyclin-Dependent Kinase Inhibitor p21 - biosynthesis / Cyclin-dependent kinase inhibitor p27 / Cyclin-Dependent Kinase Inhibitor p27 - genetics / Cyclin-Dependent Kinase Inhibitor p57 - biosynthesis / Defective mutant / Fusion protein / G1 Phase - genetics / Gene Expression / Humanities and Social Sciences / Luminescent Proteins - genetics / Mice / Mice, Transgenic / MicroRNAs - biosynthesis / multidisciplinary / Recombinant Fusion Proteins - biosynthesis / Recombinant Fusion Proteins - genetics / Resting Phase, Cell Cycle - genetics / Retinoblastoma Protein - biosynthesis / RNA-Binding Proteins - biosynthesis / RNA-Binding Proteins - genetics / Rodents / Science / Selenium-Binding Proteins - biosynthesis / Selenium-Binding Proteins - genetics / Stem cell transplantation / Stem cells / Stem Cells - cytology / Transgenic mice / Tumor Suppressor Protein p53 - biosynthesis