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Sustained synchronized neuronal network activity in a human astrocyte co-culture system
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Sustained synchronized neuronal network activity in a human astrocyte co-culture system
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Journal Article
Sustained synchronized neuronal network activity in a human astrocyte co-culture system
2016
Overview
Impaired neuronal network function is a hallmark of neurodevelopmental and neurodegenerative disorders such as autism, schizophrenia, and Alzheimer’s disease and is typically studied using genetically modified cellular and animal models. Weak predictive capacity and poor translational value of these models urge for better human derived
in vitro
models. The implementation of human induced pluripotent stem cells (hiPSCs) allows studying pathologies in differentiated disease-relevant and patient-derived neuronal cells. However, the differentiation process and growth conditions of hiPSC-derived neurons are non-trivial. In order to study neuronal network formation and (mal)function in a fully humanized system, we have established an
in vitro
co-culture model of hiPSC-derived cortical neurons and human primary astrocytes that recapitulates neuronal network synchronization and connectivity within three to four weeks after final plating. Live cell calcium imaging, electrophysiology and high content image analyses revealed an increased maturation of network functionality and synchronicity over time for co-cultures compared to neuronal monocultures. The cells express GABAergic and glutamatergic markers and respond to inhibitors of both neurotransmitter pathways in a functional assay. The combination of this co-culture model with quantitative imaging of network morphofunction is amenable to high throughput screening for lead discovery and drug optimization for neurological diseases.
Publisher
Nature Publishing Group UK,Nature Publishing Group
Subject
/ 96/100
/ 96/34
/ Action Potentials - physiology
/ Autism
/ Cell Differentiation - physiology
/ Coculture Techniques - methods
/ Humanities and Social Sciences
/ Humans
/ Induced Pluripotent Stem Cells - metabolism
/ Induced Pluripotent Stem Cells - physiology
/ Neurodevelopmental disorders
/ Neurotransmitter Agents - metabolism
/ Science
