Asset Details
Do you wish to reserve the book?
Production of inulinase, fructosyltransferase and sucrase from fungi on low-value inulin-rich substrates and their use in generation of fructose and fructo-oligosaccharides
Hey, we have placed the reservation for you!
By the way, why not check out events that you can attend while you pick your title.
Oops! Something went wrong.
Looks like we were not able to place the reservation. Kindly try again later.
Are you sure you want to remove the book from the shelf?
Production of inulinase, fructosyltransferase and sucrase from fungi on low-value inulin-rich substrates and their use in generation of fructose and fructo-oligosaccharides
Do you wish to request the book?
Production of inulinase, fructosyltransferase and sucrase from fungi on low-value inulin-rich substrates and their use in generation of fructose and fructo-oligosaccharides
Please be aware that the book you have requested cannot be checked out. If you would like to checkout this book, you can reserve another copy
We have requested the book for you!
Your request is successful and it will be processed during the Library working hours. Please check the status of your request in My Requests.
Oops! Something went wrong.
Looks like we were not able to place your request. Kindly try again later.
Journal Article
Production of inulinase, fructosyltransferase and sucrase from fungi on low-value inulin-rich substrates and their use in generation of fructose and fructo-oligosaccharides
2015
Overview
Owing to applications in the food and nutraceutical industries, inulinases, fructosyltransferases and sucrases have gained considerable attention in recent times. Twenty-five fungal strains were screened for production of these enzymes on three different media formulated using inulin-rich plant extracts prepared from asparagus root, dahlia tuber and dandelion root extract. Culture filtrates of the fungi were examined for hydrolytic activities. Fungi belonging to genus
Aspergillus,
A. niger
GNCC 2655 (11.3 U/ml),
A. awamori
MTCC 2879 (8.2 U/ml),
A. niger
ATCC 26011 (7.9 U/ml) secreted high titers of inulinase followed by
Penicillium
sp. NFCCI 2768 (2.6 U/ml) and
Penicillium citrinum
MTCC 1256 (1.1 U/ml). High sucrase activity was noticed in
A. niger
GNCC 2613 (113 U/ml) and
A. awamori
MTCC 2879 (107.8 U/ml). Analysis of end products of inulinase action by HPLC revealed that most of the enzymes were exo-inulinases liberating fructose exclusively from inulin. Five fungi,
P. citrinum
MTCC 1256,
Penicillium rugulosum
MTCC 3487,
Penicillium
sp. NFCCI 2768,
A. fumigatus
GNCC 1351 and
A. niger
ATCC 26011 however, produced a mixture of endo- and exo-inulinases liberating oligosaccharides (GF3 and GF2) along with fructose. High inulinase/sucrase yielding strains were evaluated for extracellular and intracellular hydrolytic and transfructosylating activities and intracellular enzyme profiles were found to be considerably different in terms of titers and end products.
Publisher
Springer International Publishing,Springer Nature B.V
Subject
/ Biomedical and Life Sciences
/ Chromatography, High Pressure Liquid
/ Dahlia
/ Enzymes
/ fructose
/ Functional foods & nutraceuticals
/ Fungi
/ Fungi - growth & development
/ Glycoside Hydrolases - metabolism
/ Hexosyltransferases - metabolism
/ high performance liquid chromatography
/ industry
/ inulin
/ Inulin - isolation & purification
/ Oligosaccharides - metabolism
/ Plant Extracts - isolation & purification
/ Sucrose
