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Cytokinin Signaling in Mycobacterium tuberculosis
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Journal Article
Cytokinin Signaling in Mycobacterium tuberculosis
2018
Overview
It was recently reported that the human-exclusive pathogen
Mycobacterium tuberculosis
secretes cytokinins, which had only been known as plant hormones. While cytokinins are well-established, adenine-based signaling molecules in plants, they have never been shown to participate in signal transduction in other kingdoms of life.
M. tuberculosis
is not known to interact with plants. Therefore, we tested the hypothesis that cytokinins trigger transcriptional changes within this bacterial species. Here, we show cytokinins induced the strong expression of the
M. tuberculosis
gene Rv0077c. We found that Rv0077c expression is repressed by a TetR-like transcriptional repressor, Rv0078. Strikingly, cytokinin-induced expression of Rv0077c resulted in a loss of acid-fast staining of
M. tuberculosis
. While acid-fast staining is thought to be associated with changes in the bacterial cell envelope and virulence, Rv0077c-induced loss of acid-fastness did not affect antibiotic susceptibility or attenuate bacterial growth in mice, consistent with an unaltered mycolic acid profile of Rv0077c-expressing cells. Collectively, these findings show cytokinins signal transcriptional changes that can affect
M. tuberculosis
acid-fastness and that cytokinin signaling is no longer limited to the kingdom Plantae.
IMPORTANCE
Cytokinins have only previously been known as plant hormones. The discovery that they can be used as signaling molecules outside of plants broadens the repertoire of small molecules that can potentially affect gene expression in all domains of life.
Cytokinins have only previously been known as plant hormones. The discovery that they can be used as signaling molecules outside of plants broadens the repertoire of small molecules that can potentially affect gene expression in all domains of life.
Publisher
American Society for Microbiology
Subject
/ Animals
/ Bacteria
/ Bacterial Proteins - chemistry
/ Bacterial Proteins - genetics
/ Bacterial Proteins - metabolism
/ Cloning
/ E coli
/ Enzymes
/ Female
/ Gene Expression Regulation, Bacterial - drug effects
/ Genes
/ Genomes
/ Mutation
/ Mycobacterium tuberculosis - cytology
/ Mycobacterium tuberculosis - drug effects
/ Mycobacterium tuberculosis - genetics
/ Mycobacterium tuberculosis - metabolism
/ Plasmids
/ Proteins
/ Repressor Proteins - chemistry
/ Repressor Proteins - genetics
/ Repressor Proteins - metabolism
