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TREX exposes the RNA-binding domain of Nxf1 to enable mRNA export
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TREX exposes the RNA-binding domain of Nxf1 to enable mRNA export
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TREX exposes the RNA-binding domain of Nxf1 to enable mRNA export
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Journal Article
TREX exposes the RNA-binding domain of Nxf1 to enable mRNA export
2012
Overview
The metazoan TREX complex is recruited to mRNA during nuclear RNA processing and functions in exporting mRNA to the cytoplasm. Nxf1 is an mRNA export receptor, which binds processed mRNA and transports it through the nuclear pore complex. At present, the relationship between TREX and Nxf1 is not understood. Here we show that Nxf1 uses an intramolecular interaction to inhibit its own RNA-binding activity. When the TREX subunits Aly and Thoc5 make contact with Nxf1, Nxf1 is driven into an open conformation, exposing its RNA-binding domain, allowing RNA binding. Moreover, the combined knockdown of Aly and Thoc5 markedly reduces the amount of Nxf1 bound to mRNA
in vivo
and also causes a severe mRNA export block. Together, our data indicate that TREX provides a license for mRNA export by driving Nxf1 into a conformation capable of binding mRNA.
The TREX complex and Nxf1 are involved in the export of mRNA from the nucleus but the precise molecular function of TREX is unclear. Here, the TREX components Aly and Thoc5 are shown to bind to Nxf1 resulting in a change in Nxf1 conformation that permits binding to mRNA and nuclear export.
Publisher
Nature Publishing Group UK,Nature Publishing Group
Subject
/ Active Transport, Cell Nucleus
/ Exodeoxyribonucleases - chemistry
/ Exodeoxyribonucleases - genetics
/ Exodeoxyribonucleases - metabolism
/ Humanities and Social Sciences
/ Humans
/ Nuclear Proteins - metabolism
/ Nucleocytoplasmic Transport Proteins - chemistry
/ Nucleocytoplasmic Transport Proteins - genetics
/ Nucleocytoplasmic Transport Proteins - metabolism
/ Phosphoproteins - metabolism
/ RNA-Binding Proteins - chemistry
/ RNA-Binding Proteins - genetics
/ RNA-Binding Proteins - metabolism
/ Science
