Journal Article

Low-Input, High-Resolution 5′ Terminal Filovirus RNA Sequencing with ViBE-Seq

Honko, Anna N., Olejnik, Judith, Mühlberger, Elke, McKay, Lindsay G. A., Hume, Adam J., Griffiths, Anthony, Ross, Stephen J., Cifuentes, Daniel, Connor, John H., Turcinovic, Jacquelyn

2024

Overview

Although next-generation sequencing (NGS) has been instrumental in determining the genomic sequences of emerging RNA viruses, de novo sequence determination often lacks sufficient coverage of the 5′ and 3′ ends of the viral genomes. Since the genome ends of RNA viruses contain the transcription and genome replication promoters that are essential for viral propagation, a lack of terminal sequence information hinders the efforts to study the replication and transcription mechanisms of emerging and re-emerging viruses. To circumvent this, we have developed a novel method termed ViBE-Seq (Viral Bona Fide End Sequencing) for the high-resolution sequencing of filoviral genome ends using a simple yet robust protocol with high fidelity. This technique allows for sequence determination of the 5′ end of viral RNA genomes and mRNAs with as little as 50 ng of total RNA. Using the Ebola virus and Marburg virus as prototypes for highly pathogenic, re-emerging viruses, we show that ViBE-Seq is a reliable technique for rapid and accurate 5′ end sequencing of filovirus RNA sourced from virions, infected cells, and tissue obtained from infected animals. We also show that ViBE-Seq can be used to determine whether distinct reverse transcriptases have terminal deoxynucleotidyl transferase activity. Overall, ViBE-Seq will facilitate the access to complete sequences of emerging viruses.

• Publisher: MDPI AG
• Subject: 5′ end RNA sequencing / Animals / Cell culture / DNA nucleotidylexotransferase / Ebola virus / Ebolavirus / Ebolavirus - genetics / emerging viruses / Filoviridae - genetics / Genetic aspects / Genetic transcription / Genome, Viral / Genomes / Genomics / High-Throughput Nucleotide Sequencing - methods / Humans / Infections / Invoices / Laboratories / Marburg marburgvirus / Marburg virus / Marburg virus disease / Marburgvirus - genetics / Messenger RNA / Methods / Next-generation sequencing / Nucleotide sequence / nucleotidyltransferases / Pathogens / Physiological aspects / Public health / Replication / RNA / RNA polymerase / RNA sequencing / RNA viruses / RNA, Viral - genetics / Scientific equipment and supplies industry / sequence analysis / Sequence Analysis, RNA - methods / ViBE-Seq / viral bona fide end sequencing / viral genome / Virions / Viruses / Zoonoses