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Application of next generation sequencing (NGS) for descriptive analysis of 30 genomes of Leishmania infantum isolates in Middle-North Brazil
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Application of next generation sequencing (NGS) for descriptive analysis of 30 genomes of Leishmania infantum isolates in Middle-North Brazil
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Journal Article
Application of next generation sequencing (NGS) for descriptive analysis of 30 genomes of Leishmania infantum isolates in Middle-North Brazil
2020
Overview
Visceral leishmaniasis (VL) is a life-threatening disease caused by the protozoa
Leishmania donovani
and
L. infantum
. Likely,
L. infantum
was introduced in the New World by the Iberic colonizers. Due to recent introduction, the genetic diversity is low. Access to genomic information through the sequencing of
Leishmania
isolates allows the characterization of populations through the identification and analysis of variations. Population structure information may reveal important data on disease dynamics. Aiming to describe the genetic diversity of
L. infantum
from the Middle-North, Brazil, next generation sequencing of 30
Leishmania
isolates obtained in the city of Teresina, from where the disease dispersed, was performed. The variations were categorized accordingly to the genome region and impact and provided the basis for chromosomal ploidy and population structure analysis. The results showed low diversity between the isolates and the Iberic reference genome JPCM5. Most variations were seen in non-coding regions, with modifying impact. The ploidy number analysis showed aneuploid profile. The population structure analysis revealed the presence of two
L. infantum
populations identified in Teresina. Further population genetics studies with a larger number of isolates should be performed in order to identify the genetic background associated with virulence and parasite ecology.
Publisher
Nature Publishing Group UK,Nature Publishing Group
Subject
/ Brazil
/ DNA Copy Number Variations - genetics
/ Genetics
/ Genomes
/ High-Throughput Nucleotide Sequencing - methods
/ Humanities and Social Sciences
/ Leishmania infantum - genetics
/ Leishmania infantum - isolation & purification
/ Ploidy
/ Polymorphism, Single Nucleotide - genetics
/ Protozoa
/ Science
