Asset Details
Do you wish to reserve the book?
Characterization of polydactyly chondrocytes and their use in cartilage engineering
Hey, we have placed the reservation for you!
By the way, why not check out events that you can attend while you pick your title.
Oops! Something went wrong.
Looks like we were not able to place the reservation. Kindly try again later.
Are you sure you want to remove the book from the shelf?
Characterization of polydactyly chondrocytes and their use in cartilage engineering
Do you wish to request the book?
Characterization of polydactyly chondrocytes and their use in cartilage engineering
Please be aware that the book you have requested cannot be checked out. If you would like to checkout this book, you can reserve another copy
We have requested the book for you!
Your request is successful and it will be processed during the Library working hours. Please check the status of your request in My Requests.
Oops! Something went wrong.
Looks like we were not able to place your request. Kindly try again later.
Journal Article
Characterization of polydactyly chondrocytes and their use in cartilage engineering
2019
Overview
Treating cartilage injuries and degenerations represents an open surgical challenge. The recent advances in cell therapies have raised the need for a potent off-the-shelf cell source. Intra-articular injections of TGF-β transduced polydactyly chondrocytes have been proposed as a chronic osteoarthritis treatment but despite promising results, the use of gene therapy still raises safety concerns. In this study, we characterized infant, polydactyly chondrocytes during
in vitro
expansion and chondrogenic re-differentiation. Polydactyly chondrocytes have a steady proliferative rate and re-differentiate in 3D pellet culture after up to five passages. Additionally, we demonstrated that polydactyly chondrocytes produce cartilage-like matrix in a hyaluronan-based hydrogel, namely transglutaminase cross-linked hyaluronic acid (HA-TG). We utilized the versatility of TG cross-linking to augment the hydrogels with heparin moieties. The heparin chains allowed us to load the scaffolds with TGF-β1, which induced cartilage-like matrix deposition both
in vitro
and
in vivo
in a subcutaneous mouse model. This strategy introduces the possibility to use infant, polydactyly chondrocytes for the clinical treatment of joint diseases.
Publisher
Nature Publishing Group UK,Nature Publishing Group
Subject
/ 13/107
/ 13/31
/ 13/51
/ 38
/ 38/77
/ Adult
/ Animals
/ Cartilage, Articular - cytology
/ Cartilage, Articular - metabolism
/ Cattle
/ Disease
/ Female
/ Heparin
/ Humanities and Social Sciences
/ Humans
/ Infant
/ Infants
/ Kinetics
/ Male
/ Science
/ Surgery
/ Tissue Engineering - methods
/ Transforming Growth Factor beta1 - metabolism
