Journal Article

Towards the development of phytoextract based healthy ageing cognitive booster formulation, explored through Caenorhabditis elegans model

Srivastava, Ramesh Kumar, Kumar, Sanjay, Bawankule, Dnyaneshwar Umrao, Pandey, Rakesh, Shanker, Karuna, Kumar, Narendra, Singh, Saudan, Tandon, Sudeep, Mani, Dayanandan, Khare, Puja, Singh, Dewasya Pratap, Gupta, Anil Kumar, Pandey, Taruna, Pant, Akanksha, Suresh, Ram, Kalra, Alok, Asthana, Jyotsna, Smita, Shachi Suchi, Lal, Raj Kishori, Chanda, Debabrata, Trivedi, Prabodh Kumar, Saikia, Dharmendra, Trivedi, Mashu, Trivedi, Shalini, Pal, Anirban, Maurya, Anil, Rathor, Laxmi

2022

Overview

The positive effect of herbal supplements on aging and age-related disorders has led to the evolution of natural curatives for remedial neurodegenerative diseases in humans. The advancement in aging is exceedingly linked to oxidative stress. Enhanced oxidative stress interrupts health of humans in various ways, necessitating to find stress alleviating herbal resources. Currently, minimal scientifically validated health and cognitive booster resources are available. Therefore, we explored the impact of plant extracts in different combinations on oxidative stress, life span and cognition using the multicellular transgenic humanized C. elegans, and further validated the same in Mus musculus, besides testing their safety and toxicity. In our investigations, the final product—the HACBF (healthy ageing cognitive booster formulation) thus developed was found to reduce major aging biomarkers like lipofuscin, protein carbonyl, lipid levels and enhanced activity of antioxidant enzymes. Further confirmation was done using transgenic worms and RT-PCR. The cognitive boosting activities analyzed in C. elegans and M. musculus model system were found to be at par with donepezil and L-dopa, the two drugs which are commonly used to treat Parkinson’s and Alzheimer’s diseases. In the transgenic C. elegans model system, the HACBF exhibited reduced aggregation of misfolded disease proteins α-synuclein and increased the health of nicotinic acetylcholine receptor, levels of Acetylcholine and Dopamine contents respectively, the major neurotransmitters responsible for memory, language, learning behavior and movement. Molecular studies clearly indicate that HACBF upregulated major genes responsible for healthy aging and cognitive booster activities in C. elegans and as well as in M. musculus. As such, the present herbal product thus developed may be quite useful for healthy aging and cognitive boosting activities, and more so during this covid-19 pandemic.Graphical abstract

• Publisher: Springer Nature B.V
• Subject: Acetylcholine receptors (nicotinic) / Age related diseases / Aging / Albinism / Antioxidants / Biomarkers / Carbonyl compounds / Carbonyls / Cognition / Cognition & reasoning / COVID-19 / Donepezil / Dopamine / E coli / Edema / Experiments / Hematology / Herbal medicine / Inflammation / Learning behavior / Levodopa / Life span / Lipids / Nematodes / Neurodegenerative diseases / Oxidative stress / Pandemics / Plant extracts / Proteins / Synuclein / Toxicity / Toxicity testing / Worms