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ty-5 Confers Broad-Spectrum Resistance to Geminiviruses
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Journal Article
ty-5 Confers Broad-Spectrum Resistance to Geminiviruses
2022
Overview
The selection of resistant crops is an effective method for controlling geminivirus diseases. ty-5 encodes a messenger RNA surveillance factor Pelota with a single amino acid mutation (PelotaV16G), which confers effective resistance to tomato yellow leaf curl virus (TYLCV). No studies have investigated whether ty-5 confers resistance to other geminiviruses. Here, we demonstrate that the tomato ty-5 line exhibits effective resistance to various geminiviruses. It confers resistance to two representative begomoviruses, tomato yellow leaf curl China virus/tomato yellow leaf curl China betasatellite complex and tomato leaf curl Yunnan virus. The ty-5 line also exhibits partial resistance to a curtovirus beet curly top virus. Importantly, ty-5 confers resistance to TYLCV with a betasatellite. Southern blotting and quantitative polymerase chain reaction analyses showed that significantly less DNA of these geminiviruses accumulated in the ty-5 line than in the susceptible line. Moreover, knockdown of Pelota expression converted a Nicotiana benthamiana plant from a geminivirus-susceptible host to a geminivirus-resistant host. Overall, our findings suggest that ty-5 is an important resistance gene resource for crop breeding to control geminiviruses.
Publisher
MDPI AG,MDPI
Subject
/ Cloning
/ Disease
/ DNA
/ Genes
/ Genomes
/ mRNA
/ Mutation
/ Pelota
/ Proteins
/ quantitative polymerase chain reaction
/ Tomato yellow leaf curl China virus
/ Tomato yellow leaf curl virus
/ Tomatoes
/ ty-5
/ Viruses
