Journal Article

Flesh ID: Nanopore Sequencing Combined with Offline BLAST Search for the Identification of Meat Source

Böhlken-Fascher, Susanne, Kissenkötter, Jonas, Abd El Wahed, Ahmed

2020

Overview

Detection of animal species in meat product is crucial to prevent adulterated and unnecessary contamination during processing, in addition to avoid allergy and religious consequences. Gold standard is the real-time PCR assays, which has a limited target capability. In this study, we have established a rapid sequencing protocol to identify animal species within hours. Sequencing was achieved by nanopore sequencing and data analysis via offline BLAST search. The whole procedure was conducted in a mobile suitcase lab. As per national and international regulations, the developed assay detected adulteration of pork meat with 0.1% of horse, chicken, turkey, cattle, sheep, duck, rabbit, goat, and donkey. The developed test could be used on-site as a rapid and mobile detection system to determine contamination of meat products.

• Publisher: MDPI AG,MDPI
• Subject: Accuracy / adulterated products / Allergies / Analysis / Animal species / Aquatic birds / assays / asses / Cattle / chickens / Chromosomes / Contamination / Data analysis / detection / DNA sequencing / ducks / Food / Food contamination / Food science / Genomes / goats / gold / horses / hypersensitivity / Identification / Identification and classification / International regulations / Meat / meat adulteration / Meat products / Methods / nanopore sequencing / nanopores / Nucleotide sequencing / point-of-need / Pork / protocols / quantitative polymerase chain reaction / rabbits / rapid sequencing protocol / Sheep / Software