Journal Article

Effect of fatty acid-enriched black soldier fly larvae meal combined with chitinase on the metabolic processes of Nile tilapia

Mes, Wouter, Gérardy, Mazarine, Cambier, Pierre, Kestemont, Patrick, Lalèyè, Philippe A., Tokpon, Nicole, Garigliany, Mutien-Marie, Agbohessou, Pamphile S., Mandiki, Robert, Blanquer, Aude, Lambert, Jérôme

2024

Overview

The aim of this study is to determine to what extent the addition of chitinase to black soldier fly (BSF) larval meal enriched or not with long-chain PUFA (LC-PUFA) could improve growth, protein digestion processes and gut microbial composition in Nile tilapia. Two different types of BSF meal were produced, in which larvae were reared on substrates formulated with vegetable culture substrate (VGS) or marine fish offal substrate (FOS). The BSF raised on VGS was enriched in α-linolenic acid (ALA), while that raised on FOS was enriched in ALA + EPA + DHA. Six BSF-based diets, enriched or not with chitinase, were formulated and compared with a control diet based on fishmeal and fish oil (FMFO). Two doses (D) of chitinase from Aspergillus niger (2 g and 5 g/kg feed) were added to the BSF larval diets (VGD0 and FOD0) to obtain four additional diets: VGD2, VGD5, FOD2 and FOD5. After 53 d of feeding, results showed that the BSF/FOS-based diets induced feed utilisation, protein efficiency and digestibility, as well as growth comparable to the FMFO control diet, but better than the BSF/VGS-based diets. The supplementation of chitinase to BSF/FOS increased in fish intestine the relative abundance of beneficial microbiota such as those of the Bacillaceae family. The results showed that LC-PUFA-enriched BSF meal associated with chitinase could be used as an effective alternative to fishmeal in order to improve protein digestion processes, beneficial microbiota and ultimately fish growth rate.

• Publisher: Cambridge University Press
• Subject: Animal Feed / Animal Feed/analysis / Animals / Bacteria / Chitinase / Chitinases / Cichlids / Diet / Dietary supplements / Digestibility / Digestion / Diptera / Diptera/chemistry / Enrichment / Environmental sciences & ecology / Enzymes / Fatty Acids / Fatty Acids, Unsaturated / Feed conversion / Fish meal / Fish offal / Fish oils / Growth / Gut microbiota / Hermetia illucens / Ingredients / Insects / Intestine / Larva / Larvae / Life sciences / Linolenic acid / Lipids / Marine fish / Medicine (miscellaneous) / Metabolism / Metabolism and Metabolic Studies / Microbiota / Microorganisms / Nutrition and Dietetics / Nutrition research / Oreochromis niloticus / Polyunsaturated fatty acids / Proteins / Relative abundance / Sciences de l’environnement & écologie / Sciences du vivant / Soybeans / Substrates / Tilapia / Vegetables