Journal Article

Betamethasone prevents human rhinovirus- and cigarette smoke- induced loss of respiratory epithelial barrier function

Niespodziana, Katarzyna, Cabauatan, Clarissa R., Mueller, Christian A., Eckl-Dorna, Julia, Waltl, Eva E., Resch-Marat, Yvonne, Selb, Regina, Eiwegger, Thomas, Vrtala, Susanne, Valenta, Rudolf, Niederberger, Verena

2018

Overview

The respiratory epithelium is a barrier against pathogens and allergens and a target for therapy in respiratory allergy, asthma and chronic obstructive pulmonary disease (COPD). We investigated barrier-damaging factors and protective factors by real-time measurement of respiratory cell barrier integrity. Barrier integrity to cigarette smoke extract (CSE), house dust mite (HDM) extract, interferon-γ (IFN-γ) or human rhinovirus (HRV) infection alone or in combination was assessed. Corticosteroids, lipopolysaccharide (LPS), and nasal mucus proteins were tested for their ability to prevent loss of barrier integrity. Real-time impedance-based measurement revealed different patterns of CSE-, HDM-, IFN-γ- and HRV-induced damage. When per se non-damaging concentrations of harmful factors were combined, a synergetic effect was observed only for CSE and HDM. Betamethasone prevented the damaging effect of HRV and CSE, but not damage caused by HDM or IFN-γ. Real-time impedance-based measurement of respiratory epithelial barrier function is useful to study factors, which are harmful or protective. The identification of a synergetic damaging effect of CSE and HDM as well as the finding that Betamethasone protects against HRV- and CSE-induced damage may be important for asthma and COPD.

• Publisher: Nature Publishing Group UK,Nature Publishing Group
• Subject: 13/106 / 14/63 / 631/1647/767/2201 / 692/699/1785/31 / 9/10 / Adult / Allergens / Allergies / Asthma / Betamethasone - pharmacology / Cell Survival - drug effects / Cells, Cultured / Chronic obstructive pulmonary disease / Cigarette smoke / Corticoids / Corticosteroids / Epithelial Cells - drug effects / Epithelial Cells - metabolism / Epithelium / Female / House dust / Humanities and Social Sciences / Humans / Impedance / Interferon-gamma - metabolism / Lipopolysaccharides / Lung diseases / Male / Middle Aged / multidisciplinary / Nose - cytology / Obstructive lung disease / Respiratory Mucosa - cytology / Respiratory tract / Rhinovirus / Rhinovirus - drug effects / Rhinovirus - pathogenicity / Science / Science (multidisciplinary) / Signal Transduction - drug effects / Small intestine / Smoke - adverse effects / Young Adult / γ-Interferon