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Comparison of seven single cell whole genome amplification commercial kits using targeted sequencing
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Comparison of seven single cell whole genome amplification commercial kits using targeted sequencing
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Journal Article
Comparison of seven single cell whole genome amplification commercial kits using targeted sequencing
2021
Overview
Advances in whole genome amplification (WGA) techniques enable understanding of the genomic sequence at a single cell level. Demand for single cell dedicated WGA kits (scWGA) has led to the development of several commercial kit. To this point, no robust comparison of all available kits was performed. Here, we benchmark an economical assay, comparing all commercially available scWGA kits. Our comparison is based on targeted sequencing of thousands of genomic loci, including highly mutable regions, from a large cohort of human single cells. Using this approach we have demonstrated the superiority of Ampli1 in genome coverage and of RepliG in reduced error rate. In summary, we show that no single kit is optimal across all categories, highlighting the need for a dedicated kit selection in accordance with experimental requirements.
Publisher
Nature Publishing Group UK,Nature Publishing Group,Nature Portfolio
