Journal Article

An atlas of human long non-coding RNAs with accurate 5′ ends

Denisenko, Elena, Testa, Alison C., Harshbarger, Jayson, Mendez, Mickaël, Heutink, Peter, Alam, Tanvir, Carninci, Piero, Lizio, Marina, Arner, Erik, Poulsen, Thomas M., Bertin, Nicolas, Forrest, Alistair R. R., Noma, Shohei, Babina, Magda, Schmeier, Sebastian, Abugessaisa, Imad, Djebali, Sarah, Lassmann, Timo, Nakamura, Yukio, Hon, Chung-Chau, Kojima, Soichi, Suzuki, Harukazu, Medvedeva, Yulia A., Tang, Dave, Wells, Christine A., Hayashizaki, Yoshihide, Kasukawa, Takeya, Lipovich, Leonard, Daub, Carsten O., de Hoon, Michiel J. L., Yip, Chi-Wai, Hasegawa, Akira, Gough, Julian, Burroughs, A. Maxwell, Rackham, Owen J. L., Kawaji, Hideya, Ramilowski, Jordan A., Severin, Jessica, Itoh, Masayoshi

2017

Overview

Long non-coding RNAs (lncRNAs) are largely heterogeneous and functionally uncharacterized. Here, using FANTOM5 cap analysis of gene expression (CAGE) data, we integrate multiple transcript collections to generate a comprehensive atlas of 27,919 human lncRNA genes with high-confidence 5′ ends and expression profiles across 1,829 samples from the major human primary cell types and tissues. Genomic and epigenomic classification of these lncRNAs reveals that most intergenic lncRNAs originate from enhancers rather than from promoters. Incorporating genetic and expression data, we show that lncRNAs overlapping trait-associated single nucleotide polymorphisms are specifically expressed in cell types relevant to the traits, implicating these lncRNAs in multiple diseases. We further demonstrate that lncRNAs overlapping expression quantitative trait loci (eQTL)-associated single nucleotide polymorphisms of messenger RNAs are co-expressed with the corresponding messenger RNAs, suggesting their potential roles in transcriptional regulation. Combining these findings with conservation data, we identify 19,175 potentially functional lncRNAs in the human genome. A catalogue of human long non-coding RNA genes and their expression profiles across samples from major human primary cell types, tissues and cell lines. A catalogue of long non-coding RNAs Alistair Forrest, Piero Carninci and colleagues of the FANTOM Consortium provide a catalogue of human long non-coding RNA (lncRNA) genes and their expression profiles across samples from human primary cell types, tissues and cell lines. They used combined analyses of multiple data sets to identify 27,919 lncRNA genes with high-confidence 5′ ends, as well as a subset of 19,175 potentially functional lncRNA loci. The lncRNA catalogue and annotations are available through an open web resource.

• Publisher: Nature Publishing Group UK,Nature Publishing Group
• Subject: 631/208/191/2018 / 631/208/726/2102 / 631/337/384/2568 / Biosynthesis / Cells, Cultured / Conserved Sequence - genetics / Databases, Genetic / Datasets as Topic / Enhancer Elements, Genetic - genetics / Epigenesis, Genetic / Gene Expression Profiling / Gene Expression Regulation / Gene mapping / Genome, Human - genetics / Genome-Wide Association Study / Genomics / Humanities and Social Sciences / Humans / Internet / Life Sciences / Molecular Sequence Annotation / multidisciplinary / Organ Specificity - genetics / Polymorphism, Single Nucleotide / Promoter Regions, Genetic - genetics / Quantitative Trait Loci - genetics / RNA polymerase / RNA Stability / RNA, Long Noncoding - chemistry / RNA, Long Noncoding - genetics / RNA, Messenger - genetics / Science / Transcriptome - genetics