Journal Article

Inflammation boosts bacteriophage transfer between Salmonella spp

Diard, Mederic, Sellin, Mikael E, Bakkeren, Erik, Swiss National Foundation (SNF) [31003053074, Sinergia CRSII_154414/1]; Novartis FreeNovation program; Swedish Research Council [2012-262: 2015-00635]; SNF Ambizione fellowship [PZ00P3_136712]; SNF Muse Heim-Voglin stipend; ETH Excellence Scholarship and Opportunity Program stipend, Université Paris-Saclay, Laboratoire Jean Perrin (LJP) ; Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut de Biologie Paris Seine (IBPS) ; Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), Department of Environmental Systems Science ; Eidgenössische Technische Hochschule - Swiss Federal Institute of Technology [Zürich] (ETH Zürich), MICrobiologie de l'ALImentation au Service d

2017

Overview

Bacteriophage transfer (lysogenic conversion) promotes bacterial virulence evolution. There is limited understanding of the factors that determine lysogenic conversion dynamics within infected hosts. Amurine Salmonella Typhimurium (S.Tm) diarrhea model was used to study the transfer of SopEF, a prophage from S.Tm SL1344, to S.Tm ATCC14028S. Gut inflammation and enteric disease triggered >55% lysogenic conversion of ATCC14028S within 3 days. Without inflammation, SopEF transfer was reduced by up to 10(5)-fold. This was because inflammation (e.g., reactive oxygen species, reactive nitrogen species, hypochlorite) triggers the bacterial SOS response, boosts expression of the phage antirepressor Tum, and thereby promotes free phage production and subsequent transfer. Mucosal vaccination prevented a dense intestinal S.Tm population from inducing inflammation and consequently abolished SopEF transfer. Vaccination may be a general strategy for blocking pathogen evolution that requires disease-driven transfer of temperate bacteriophages.

• Publisher: American Association for the Advancement of Science (AAAS),CCSD,American Association for the Advancement of Science,The American Association for the Advancement of Science
• Subject: Animals / Bacteria / Bacteriophages / Conversion / Diarrhea - microbiology / Diarrhea - pathology / Disease Models, Animal / Enteritis - microbiology / Enteritis - prevention & control / Evolution / Immune response / Immunization / Infections / Inflammation / Inflammation - microbiology / Inflammation - prevention & control / Intestines - microbiology / Life Sciences / Lysogeny / Mathematical models / Mice / Mice, Inbred C57BL / Parasites / Pathogens / Phage / Phages / Reactive nitrogen species / Reactive oxygen species / Salmonella / Salmonella Phages - genetics / Salmonella Phages - pathogenicity / Salmonella typhimurium / Salmonella typhimurium - pathogenicity / Salmonella typhimurium - virology / SOS Response (Genetics) / Vaccination / Vaccines / Viral Proteins - metabolism / Virulence
• ISBN: 0003963512000