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Mapping mitochondrial morphology and function: COX-SBFSEM reveals patterns in mitochondrial disease
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Mapping mitochondrial morphology and function: COX-SBFSEM reveals patterns in mitochondrial disease
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Journal Article
Mapping mitochondrial morphology and function: COX-SBFSEM reveals patterns in mitochondrial disease
2025
Overview
Mitochondria play a crucial role in maintaining cellular health. It is interesting that the shape of mitochondria can vary depending on the type of cell, mitochondrial function, and other cellular conditions. However, there are limited studies that link functional assessment with mitochondrial morphology evaluation at high magnification, even fewer that do so in situ and none in human muscle biopsies. Therefore, we have developed a method which combines functional assessment of mitochondria through Cytochrome c Oxidase (COX) histochemistry, with a 3D electron microscopy (EM) technique, serial block-face scanning electron microscopy (SBFSEM). Here we apply COX-SBFSEM to muscle samples from patients with single, large-scale mtDNA deletions, a cause of mitochondrial disease. These deletions cause oxidative phosphorylation deficiency, which can be observed through changes in COX activity. One of the main advantages of combining 3D-EM with the COX reaction is the ability to look at how per-mitochondrion oxidative phosphorylation status is spatially distributed within muscle fibres. Here we show a robust spatial pattern in COX-positive and intermediate-fibres and that the spatial pattern is less clear in COX-deficient fibres.
Innovative COX-SBFSEM method integrates mitochondrial function and morphology assessment to study muscle biopsies from patients with mtDNA deletions, revealing spatial patterns in oxidative phosphorylation within muscle fibers.
Publisher
Nature Publishing Group UK,Nature Publishing Group,Nature Portfolio
Subject
/ 13
/ Biomedical and Life Sciences
/ Biopsy
/ DNA, Mitochondrial - genetics
/ DNA, Mitochondrial - metabolism
/ Electron Transport Complex IV - metabolism
/ Female
/ Humans
/ Male
/ Microscopy, Electron, Scanning
/ Mitochondria - ultrastructure
/ Mitochondrial Diseases - genetics
/ Mitochondrial Diseases - metabolism
