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Utility of gel-free, label-free shotgun proteomics approaches to investigate microorganisms
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Utility of gel-free, label-free shotgun proteomics approaches to investigate microorganisms
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Utility of gel-free, label-free shotgun proteomics approaches to investigate microorganisms
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Journal Article
Utility of gel-free, label-free shotgun proteomics approaches to investigate microorganisms
2011
Overview
This review will examine the current situation with label-free, quantitative, shotgun-oriented proteomics technology and discuss the advantages and limitations associated with its capability in capturing and quantifying large portions of proteomes of microorganisms. Such an approach allows: 1. comparisons between physiological or genetic states of organisms at the protein level, 2. painting of proteomic data onto genome data-based metabolic maps, 3. enhancement of the utility of genomic data and finally 4. surveying of non-genome sequenced microorganisms by taking advantage of available inferred protein data in order to gain new insights into strain-dependent metabolic or physiological capacities. The technology essentially is a powerful addition to systems biology with a capacity to be used to ask hypothesis-driven top-down questions or for more empirical bottom-up exploration. [PUBLICATION ABSTRACT]
