Asset Details
Do you wish to reserve the book?
Identification of T6SS-dependent effector and immunity proteins by Tn-seq in Vibrio cholerae
Hey, we have placed the reservation for you!
By the way, why not check out events that you can attend while you pick your title.
Oops! Something went wrong.
Looks like we were not able to place the reservation. Kindly try again later.
Are you sure you want to remove the book from the shelf?
Identification of T6SS-dependent effector and immunity proteins by Tn-seq in Vibrio cholerae
Do you wish to request the book?
Identification of T6SS-dependent effector and immunity proteins by Tn-seq in Vibrio cholerae
Please be aware that the book you have requested cannot be checked out. If you would like to checkout this book, you can reserve another copy
We have requested the book for you!
Your request is successful and it will be processed during the Library working hours. Please check the status of your request in My Requests.
Oops! Something went wrong.
Looks like we were not able to place your request. Kindly try again later.
Journal Article
Identification of T6SS-dependent effector and immunity proteins by Tn-seq in Vibrio cholerae
2013
Overview
Type VI protein secretion system (T6SS) is important for bacterial competition through contact-dependent killing of competitors. T6SS delivers effectors to neighboring cells and corresponding antagonistic proteins confer immunity against effectors that are delivered by sister cells. Although T6SS has been found in more than 100 gram-negative bacteria including many important human pathogens, few T6SS-dependent effector and immunity proteins have been experimentally determined. Here we report a high-throughput approach using transposon mutagenesis and deep sequencing (Tn-seq) to identify T6SS immunity proteins in Vibrio cholerae . Saturating transposon mutagenesis was performed in wild type and a T6SS null mutant. Genes encoding immunity proteins were predicted to be essential in the wild type but dispensable in the T6SS mutant. By comparing the relative abundance of each transposon mutant in the mutant library using deep sequencing, we identified three immunity proteins that render protection against killing by T6SS predatory cells. We also identified their three cognate T6SS-secreted effectors and show these are important for not only antibacterial and antieukaryotic activities but also assembly of T6SS apparatus. The lipase and muramidase T6SS effectors identified in this study underscore the diversity of T6SS-secreted substrates and the distinctly different mechanisms that target these for secretion by the dynamic T6SS organelle.
Publisher
National Academy of Sciences,National Acad Sciences
Subject
/ Bacteria
/ Bacterial Proteins - genetics
/ Bacterial Proteins - immunology
/ Bacterial Secretion Systems - genetics
/ Bacterial Secretion Systems - immunology
/ Cells
/ Dictyostelium - growth & development
/ Dictyostelium - microbiology
/ DNA Transposable Elements - genetics
/ Genes
/ High-Throughput Nucleotide Sequencing
/ humans
/ Immunity
/ lysozyme
/ Microbial Interactions - genetics
/ Microbial Interactions - immunology
/ Mutants
/ Mutation
/ Proteins
