Journal Article

Insulin-Like Growth Factors Are Expressed in the Taste System, but Do Not Maintain Adult Taste Buds

Biggs, Bradley T., Tang, Tao, Krimm, Robin F.

2016

Overview

Growth factors regulate cell growth and differentiation in many tissues. In the taste system, as yet unknown growth factors are produced by neurons to maintain taste buds. A number of growth factor receptors are expressed at greater levels in taste buds than in the surrounding epithelium and may be receptors for candidate factors involved in taste bud maintenance. We determined that the ligands of eight of these receptors were expressed in the E14.5 geniculate ganglion and that four of these ligands were expressed in the adult geniculate ganglion. Of these, the insulin-like growth factors (IGF1, IGF2) were expressed in the ganglion and their receptor, insulin-like growth factor receptor 1 (IGF1R), were expressed at the highest levels in taste buds. To determine whether IGF1R regulates taste bud number or structure, we conditionally eliminated IGF1R from the lingual epithelium of mice using the keratin 14 (K14) promoter (K14-Cre::Igf1rlox/lox). While K14-Cre::Igf1rlox/lox mice had significantly fewer taste buds at P30 compared with control mice (Igf1rlox/lox), this difference was not observed by P80. IGF1R removal did not affect taste bud size or cell number, and the number of phospholipase C β2- (PLCβ2) and carbonic anhydrase 4- (Car4) positive taste receptor cells did not differ between genotypes. Taste buds at the back of the tongue fungiform taste field were larger and contained more cells than those at the tongue tip, and these differences were diminished in K14-Cre::Igf1rlox/lox mice. The epithelium was thicker at the back versus the tip of the tongue, and this difference was also attenuated in K14-Cre::Igf1rlox/lox mice. We conclude that, although IGFs are expressed at high levels in the taste system, they likely play little or no role in maintaining adult taste bud structure. IGFs have a potential role in establishing the initial number of taste buds, and there may be limits on epithelial thickness in the absence of IGF1R signaling.

• Publisher: Public Library of Science,Public Library of Science (PLoS)
• Subject: Animal tissues / Animals / Biology and Life Sciences / Brain-derived neurotrophic factor / Carbonic anhydrase / Carbonic anhydrases / Cell number / Cell size / Epithelium / Geniculate Ganglion - metabolism / Genotypes / Growth factor receptors / Growth factors / Immunohistochemistry / Insulin / Insulin-like growth factor I / Insulin-like growth factor I receptors / Insulin-like growth factor II / Insulin-like growth factors / Keratin / Kinases / Laboratory animals / Laser Capture Microdissection / Ligands / Lingual epithelium / Liquid oxygen / Medicine and Health Sciences / Mice / Neurobiology / Neurology / Neurosciences / Phospholipase / Phospholipase C / Real-Time Polymerase Chain Reaction / Receptor, IGF Type 1 - metabolism / Receptor, IGF Type 2 - metabolism / Receptors / Receptors, Somatomedin - metabolism / Rodents / Social Sciences / Somatomedins - metabolism / Taste / Taste buds / Taste Buds - metabolism / Taste receptor neurons / Tongue / Tongue - metabolism