Journal Article

Lack of Adiponectin Drives Hyperosteoclastogenesis in Lipoatrophic Mice

Blin-Wakkach, Claudine, Madel, Maria-Bernadette, Bonnet, Nicolas, Desvergne, Béatrice, Winkler, Carine, Toffoli, Barbara, Ferrari, Serge, Schiffrin, Mariano, Pierroz, Dominique D., Pochon, Cécile, Fu, He, Jouzeau, Jean-Yves, Gilardi, Federica, Wilson, Anne, Taïeb, Mahdia, Moulin, David

2021

Overview

Long bones from mammals host blood cell formation and contain multiple cell types, including adipocytes. Physiological functions of bone marrow adipocytes are poorly documented. Herein, we used adipocyte-deficient PPARγ-whole body null mice to investigate the consequence of total adipocyte deficiency on bone homeostasis in mice. We first highlighted the dual bone phenotype of PPARγ null mice: one the one hand, the increased bone formation and subsequent trabecularization extending in the long bone diaphysis, due to the well-known impact of PPARγ deficiency on osteoblasts formation and activity; on the other hand, an increased osteoclastogenesis in the cortical bone. We then further explored the cause of this unexpected increased osteoclastogenesis using two independent models of lipoatrophy, which recapitulated this phenotype. This demonstrates that hyperosteoclastogenesis is not intrinsically linked to PPARγ deficiency, but is a consequence of the total lipodystrophy. We further showed that adiponectin, a cytokine produced by adipocytes and mesenchymal stromal cells is a potent inhibitor of osteoclastogenesis in vitro and in vivo . Moreover, pharmacological activation of adiponectin receptors by the synthetic agonist AdipoRon inhibited mature osteoclast activity both in mouse and human cells by blocking podosome formation through AMPK activation. Finally, we demonstrated that AdipoRon treatment blocks bone erosion in vivo in a murine model of inflammatory bone loss, providing potential new approaches to treat osteoporosis.

• Publisher: Frontiers Media SA,Frontiers media,Frontiers Media S.A
• Subject: Adipocytes / Adiponectin / AMPK / Animal biology / Animal models / Body fat / Bone growth / Bone loss / Bone marrow / bone marrow adiposity / Bone turnover / Cell and Developmental Biology / Cortical bone / cortical bone porosity / Diaphysis / Females / Genotype & phenotype / Homeostasis / Life Sciences / Lipoatrophy / Lipodystrophy / Long bone / Mesenchymal stem cells / osteoclast / Osteoclastogenesis / Osteogenesis / Osteoporosis / Peroxisome proliferator-activated receptors / Phenotypes / Phosphatase / Stromal cells / Women