Journal Article

Bioevaluation of superparamagnetic iron oxide nanoparticles (SPIONs) functionalized with dihexadecyl phosphate (DHP)

Mieloch, Adam Aron, Rozwadowska, Natalia, Rybka, Jakub Dalibor, Giersig, Michael, Żurawek, Magdalena

2020

Overview

Superparamagnetic iron oxide nanoparticles (SPIONs) have been investigated for wide variety of applications. Their unique properties render them highly applicable as MRI contrast agents, in magnetic hyperthermia or targeted drug delivery. SPIONs surface properties affect a whole array of parameters such as: solubility, toxicity, stability, biodistribution etc. Therefore, progress in the field of SPIONs surface functionalization is crucial for further development of therapeutic or diagnostic agents. In this study, SPIONs were synthesized by thermal decomposition of iron (III) acetylacetonate Fe(acac) 3 and functionalized with dihexadecyl phosphate (DHP) via phase transfer. Bioactivity of the SPION-DHP was assessed on SW1353 and TCam-2 cancer derived cell lines. The following test were conducted: cytotoxicity and proliferation assay, reactive oxygen species (ROS) assay, SPIONs uptake ( via Iron Staining and ICP-MS), expression analysis of the following genes: alkaline phosphatase ( ALPL ); ferritin light chain ( FTL ); serine/threonine protein phosphatase 2A ( PP2A ); protein tyrosine phosphatase non-receptor type 11 ( PTPN11 ); transferrin receptor 1 ( TFRC ) via RT-qPCR. SPION-DHP nanoparticles were successfully obtained and did not reveal significant cytotoxicity in the range of tested concentrations. ROS generation was elevated, however not correlated with the concentrations. Gene expression profile was slightly altered only in SW1353 cells.

• Publisher: Nature Publishing Group UK,Nature Publishing Group
• Subject: 14/5 / 38/77 / 631/61/350/354 / 639/301/357/354 / Alkaline phosphatase / Alkaline Phosphatase - genetics / Alkaline Phosphatase - metabolism / Antigens, CD - genetics / Antigens, CD - metabolism / Apoferritins - genetics / Apoferritins - metabolism / Biological activity / Cell Line, Tumor / Cell Proliferation - drug effects / Cell Survival - drug effects / Chondrocytes - cytology / Chondrocytes - drug effects / Chondrocytes - metabolism / Cytotoxicity / Diagnostic agents / Drug delivery / Epithelial Cells - cytology / Epithelial Cells - drug effects / Epithelial Cells - metabolism / Ferric Compounds - chemistry / Ferric Compounds - pharmacology / Ferritin / Gene expression / Gene Expression Regulation - drug effects / Humanities and Social Sciences / Humans / Hydroxybutyrates - chemistry / Hyperthermia / Iron oxides / Magnetic resonance imaging / Magnetite Nanoparticles - chemistry / multidisciplinary / Nanoparticles / Organophosphates - chemistry / Pentanones - chemistry / Phosphatase / Phosphoprotein phosphatase / Protein phosphatase / Protein Phosphatase 2 - genetics / Protein Phosphatase 2 - metabolism / Protein Tyrosine Phosphatase, Non-Receptor Type 11 - genetics / Protein Tyrosine Phosphatase, Non-Receptor Type 11 - metabolism / Protein-tyrosine-phosphatase / Reactive oxygen species / Reactive Oxygen Species - metabolism / Receptors, Transferrin - genetics / Receptors, Transferrin - metabolism / Science / Science (multidisciplinary) / Serine / Succimer - chemistry / Surface properties / Thermal decomposition / Threonine / Transferrins / Tumor cell lines