Journal Article

Global and cell-type gene expression profiles in tomato plants colonized by an arbuscular mycorrhizal fungus

Accotto, Gian Paolo, Miozzi, Laura, Catoni, Marco, Lanfranco, Luisa, Fiorilli, Valentina, Novero, Mara

2009

Overview

Arbuscular mycorrhizal symbiosis develops in roots; extensive cellular reorganizations and specific metabolic changes occur, which are mirrored by local and systemic changes in the transcript profiles. A TOM2 microarray (c. 12 000 probes) has been used to obtain an overview of the transcriptional changes that are triggered in Solanum lycopersicum roots and shoots, as a result of colonization by the arbuscular mycorrhizal fungus Glomus mosseae. The cell-type expression profile of a subset of genes was monitored, using laser microdissection, to identify possible plant determinants of arbuscule development,. Microarrays revealed 362 up-regulated and 293 down-regulated genes in roots. Significant gene modulation was also observed in shoots: 85 up- and 337 down-regulated genes. The most responsive genes in both organs were ascribed to primary and secondary metabolism, defence and response to stimuli, cell organization and protein modification, and transcriptional regulation. Six genes, preferentially expressed in arbusculated cells, were identified. A comparative analysis only showed a limited overlap with transcript profiles identified in mycorrhizal roots of Medicago truncatula, probably as a consequence of the largely nonoverlapping probe sets on the microarray tools used. The results suggest that auxin and abscisic acid metabolism are involved in arbuscule formation and/or functioning.

• Publisher: Oxford, UK : Blackwell Publishing Ltd,Blackwell Publishing,Blackwell Publishing Ltd
• Subject: abscisic acid / Abscisic Acid - metabolism / arbuscular mycorrhizal symbiosis / Arbuscular mycorrhizas / arbusculated cells / auxins / Cell walls / Down regulation / Fungi / Gene Expression / Gene Expression Profiling / gene expression regulation / Gene Expression Regulation, Plant / Genes / Genes, Plant / genetics / Glomeromycota / Glomus mosseae / Indoleacetic Acids / Indoleacetic Acids - metabolism / laser / Lycopersicon esculentum / Lycopersicon esculentum - genetics / Lycopersicon esculentum - metabolism / Medicago / Medicago - genetics / Medicago - metabolism / Medicago truncatula / metabolism / microarray / Microarray Analysis / microarray technology / microdissection / Mycorrhizae / Mycorrhizae - genetics / Mycorrhizae - metabolism / mycorrhizal fungi / Plant Proteins / Plant Proteins - genetics / Plant Proteins - metabolism / Plant Roots / Plant Roots - genetics / Plant Roots - metabolism / Plant Shoots / Plant Shoots - genetics / Plant Shoots - metabolism / Plant Structures / Plant Structures - genetics / Plant Structures - metabolism / Plants / RNA / roots / shoots / Solanum lycopersicum / Symbiosis / tomato / tomatoes / transcription (genetics) / Up regulation / vesicular arbuscular mycorrhizae