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Mechanism of oxidative DNA damage induction in a strict anaerobe, Prevotella melaninogenica
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Mechanism of oxidative DNA damage induction in a strict anaerobe, Prevotella melaninogenica
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Journal Article
Mechanism of oxidative DNA damage induction in a strict anaerobe, Prevotella melaninogenica
2000
Overview
Abstract
We investigated the mechanism of the oxidative DNA damage induction by exposure to O2 in Prevotella melaninogenica, a strict anaerobe. Flow cytometry with hydroethidine and dichlorofluorescein diacetate showed that O2 exposure generated O2●− and H2O2. Results of electron spin resonance with α-(4-pyridyl-1-oxide)-N-tert-butylnitrone and ethanol showed that O2 exposure also induced ●OH radical generation in P. melaninogenica loaded with FeCl2 but not in samples without FeCl2 loading. In P. melaninogenica, O2 exposure increased 8-hydroxydeoxyguanosine (8OHdG), typical of oxidative DNA damage. Catalase inhibited the increase, but the ●OH radical scavengers did not. Phenanthroline, a membrane-permeable Fe and Cu chelator, increased the 8OHdG induction. In FeCl2-loaded samples, induction of 8OHdG decreased. Addition of H2O2 markedly increased 8OHdG levels. These results indicate that in P. melaninogenica, exposure to O2 generated and accumulated O2●− and H2O2, and that a crypto-OH radical generated through H2O2 was the active species in the 8OHdG induction.
Publisher
Oxford University Press (OUP),Blackwell Publishing Ltd,Blackwell,Oxford University Press
Subject
/ Action of physical and chemical agents on bacteria
/ Biological and medical sciences
/ Catalase
/ Damage
/ Deoxyguanosine - analogs & derivatives
/ DNA
/ Electron paramagnetic resonance
/ Electron Spin Resonance Spectroscopy
/ Electron Spin Resonance Spectroscopy - methods
/ Ethanol
/ Exposure
/ Fundamental and applied biological sciences. Psychology
/ Hydrogen Peroxide - metabolism
/ Hydroxyl Radical - metabolism
/ Oxygen
/ Prevotella melaninogenica - drug effects
/ Prevotella melaninogenica - genetics
/ Prevotella melaninogenica - physiology
