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Pharmacological manipulation of blood and lymphatic vascularization in ex vivo–cultured mouse embryos
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Pharmacological manipulation of blood and lymphatic vascularization in ex vivo–cultured mouse embryos
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Journal Article
Pharmacological manipulation of blood and lymphatic vascularization in ex vivo–cultured mouse embryos
2012
Overview
Formation of new blood and lymphatic vessels is involved in many physiological and pathological processes, including organ and tumor growth, cancer cell metastasis, fluid drainage and lymphedema. Therefore, the ability to manipulate vascularization in a mammalian system is of particular interest to researchers. Here we describe a method for pharmacological manipulation of
de novo
and sprouting blood and lymphatic vascular development in
ex vivo
–cultured mouse embryos. The described protocol can also be used to evaluate the properties of pharmacological agents in growing mammalian tissues and to manipulate other developmental processes. The whole procedure, from embryo isolation to image quantification, takes 3–5 d, depending on the analysis and age of the embryos.
Publisher
Nature Publishing Group UK,Nature Publishing Group
Subject
/ 13/51
/ 14/19
/ 14/63
/ 631/136
/ 64/60
/ 65/1
/ Animals
/ Blood
/ Computational Biology/Bioinformatics
/ Embryo, Mammalian - blood supply
/ Embryo, Mammalian - drug effects
/ Embryos
/ Lymphatic System - blood supply
/ Lymphatic System - drug effects
/ Mammals
/ Methods
/ Mice
/ Neovascularization, Physiologic - drug effects
/ protocol
/ Vascular Endothelial Growth Factor Receptor-2 - antagonists & inhibitors
