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Molecular Detection of Various Non-Seasonal, Zoonotic Influenza Viruses Using BioFire FilmArray and GenXpert Diagnostic Platforms
by
Chestley, Taeyo
, Hart, Laura
, Bastien, Nathalie
, Meek, Breanna
, Ranadheera, Charlene
, Perez, Orlando
, Berhane, Yohannes
, Johnson, Morgan
in
Analysis
/ Animals
/ Avian flu
/ Avian influenza
/ Bird populations
/ Birds - virology
/ DNA microarrays
/ Environmental aspects
/ Epidemiology
/ Genetic aspects
/ Health aspects
/ Humans
/ Infections
/ Influenza A
/ Influenza A virus - classification
/ Influenza A virus - genetics
/ Influenza A virus - isolation & purification
/ Influenza A Virus, H5N1 Subtype - genetics
/ Influenza A Virus, H5N1 Subtype - isolation & purification
/ Influenza in Birds - diagnosis
/ Influenza in Birds - virology
/ Influenza, Human - diagnosis
/ Influenza, Human - virology
/ Laboratories
/ Molecular Diagnostic Techniques - methods
/ Orthomyxoviridae Infections - diagnosis
/ Orthomyxoviridae Infections - virology
/ pandemic preparedness
/ Pandemics
/ Pathogens
/ point of care testing
/ Prevention
/ Public health
/ Respiratory syncytial virus
/ Risk factors
/ Saliva
/ Severe acute respiratory syndrome coronavirus 2
/ Swine flu
/ Viruses
/ Zoonoses
/ Zoonoses - diagnosis
/ Zoonoses - virology
/ zoonotic influenza
2025
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Molecular Detection of Various Non-Seasonal, Zoonotic Influenza Viruses Using BioFire FilmArray and GenXpert Diagnostic Platforms
by
Chestley, Taeyo
, Hart, Laura
, Bastien, Nathalie
, Meek, Breanna
, Ranadheera, Charlene
, Perez, Orlando
, Berhane, Yohannes
, Johnson, Morgan
in
Analysis
/ Animals
/ Avian flu
/ Avian influenza
/ Bird populations
/ Birds - virology
/ DNA microarrays
/ Environmental aspects
/ Epidemiology
/ Genetic aspects
/ Health aspects
/ Humans
/ Infections
/ Influenza A
/ Influenza A virus - classification
/ Influenza A virus - genetics
/ Influenza A virus - isolation & purification
/ Influenza A Virus, H5N1 Subtype - genetics
/ Influenza A Virus, H5N1 Subtype - isolation & purification
/ Influenza in Birds - diagnosis
/ Influenza in Birds - virology
/ Influenza, Human - diagnosis
/ Influenza, Human - virology
/ Laboratories
/ Molecular Diagnostic Techniques - methods
/ Orthomyxoviridae Infections - diagnosis
/ Orthomyxoviridae Infections - virology
/ pandemic preparedness
/ Pandemics
/ Pathogens
/ point of care testing
/ Prevention
/ Public health
/ Respiratory syncytial virus
/ Risk factors
/ Saliva
/ Severe acute respiratory syndrome coronavirus 2
/ Swine flu
/ Viruses
/ Zoonoses
/ Zoonoses - diagnosis
/ Zoonoses - virology
/ zoonotic influenza
2025
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Molecular Detection of Various Non-Seasonal, Zoonotic Influenza Viruses Using BioFire FilmArray and GenXpert Diagnostic Platforms
by
Chestley, Taeyo
, Hart, Laura
, Bastien, Nathalie
, Meek, Breanna
, Ranadheera, Charlene
, Perez, Orlando
, Berhane, Yohannes
, Johnson, Morgan
in
Analysis
/ Animals
/ Avian flu
/ Avian influenza
/ Bird populations
/ Birds - virology
/ DNA microarrays
/ Environmental aspects
/ Epidemiology
/ Genetic aspects
/ Health aspects
/ Humans
/ Infections
/ Influenza A
/ Influenza A virus - classification
/ Influenza A virus - genetics
/ Influenza A virus - isolation & purification
/ Influenza A Virus, H5N1 Subtype - genetics
/ Influenza A Virus, H5N1 Subtype - isolation & purification
/ Influenza in Birds - diagnosis
/ Influenza in Birds - virology
/ Influenza, Human - diagnosis
/ Influenza, Human - virology
/ Laboratories
/ Molecular Diagnostic Techniques - methods
/ Orthomyxoviridae Infections - diagnosis
/ Orthomyxoviridae Infections - virology
/ pandemic preparedness
/ Pandemics
/ Pathogens
/ point of care testing
/ Prevention
/ Public health
/ Respiratory syncytial virus
/ Risk factors
/ Saliva
/ Severe acute respiratory syndrome coronavirus 2
/ Swine flu
/ Viruses
/ Zoonoses
/ Zoonoses - diagnosis
/ Zoonoses - virology
/ zoonotic influenza
2025
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Molecular Detection of Various Non-Seasonal, Zoonotic Influenza Viruses Using BioFire FilmArray and GenXpert Diagnostic Platforms
Journal Article
Molecular Detection of Various Non-Seasonal, Zoonotic Influenza Viruses Using BioFire FilmArray and GenXpert Diagnostic Platforms
2025
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Overview
Since 2020, the Gs/Gd H5N1 influenza virus (clade 2.3.4.4b) has established itself within wild bird populations across Asia, Europe, and the Americas, causing outbreaks in wild mammals, commercial poultry, and dairy farms. The impacts on the bird populations and the agricultural industry has been significant, requiring a One Health approach to enhanced surveillance in both humans and animals. To support pandemic preparedness efforts, we evaluated the Cepheid Xpert Xpress CoV-2/Flu/RSV plus kit and the BioFire Respiratory 2.1 Panel for their ability to detect the presence of non-seasonal, zoonotic influenza A viruses, including circulating H5N1 viruses from clade 2.3.4.4b. Both assays effectively detected the presence of influenza virus in clinically-contrived nasal swab and saliva specimens at low concentrations. The results generated using the Cepheid Xpert Xpress CoV-2/Flu/RSV plus kit and the BioFire Respiratory 2.1 Panel, in conjunction with clinical and epidemiological findings provide valuable diagnostic findings that can strengthen pandemic preparedness and surveillance initiatives.
Publisher
MDPI AG,MDPI
Subject
/ Animals
/ Humans
/ Influenza A virus - classification
/ Influenza A virus - genetics
/ Influenza A virus - isolation & purification
/ Influenza A Virus, H5N1 Subtype - genetics
/ Influenza A Virus, H5N1 Subtype - isolation & purification
/ Influenza in Birds - diagnosis
/ Influenza in Birds - virology
/ Influenza, Human - diagnosis
/ Molecular Diagnostic Techniques - methods
/ Orthomyxoviridae Infections - diagnosis
/ Orthomyxoviridae Infections - virology
/ Saliva
/ Severe acute respiratory syndrome coronavirus 2
/ Viruses
/ Zoonoses
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