Journal Article

Peroxisome-based metabolic engineering for biomanufacturing and agriculture

Jin, Yijun, Dai, Huaxin, Lian, Jiazhang, Song, Shuyan, Hu, Jianping, Ye, Cuifang, Pan, Ronghui

2024

Overview

Peroxisomes are favorable platforms for organelle engineering, featuring abundant endogenous metabolites, optimal membrane permeability, frequent contacts with other organelles, efficient protein targeting signals, and many known organelle proliferation factors.Yeast peroxisome engineering has produced valuable biomolecules, such as biopolyesters, biofuels, terpenoids, alkaloids, antibiotics, and phytohormones.Plant peroxisome engineering has great potential in biomanufacturing and agriculture and adds values to improving environmental sustainability and food security.Approaches for improving the performance of heterologous pathways in peroxisomes include increasing peroxisomal abundance, coupling multiple subcellular compartments, maximizing precursor and cofactor supplies, uncovering the rate-limiting steps, and peroxisomal surface display of enzymes. Subcellular compartmentalization of metabolic pathways plays a crucial role in metabolic engineering. The peroxisome has emerged as a highly valuable and promising compartment for organelle engineering, particularly in the fields of biological manufacturing and agriculture. In this review, we summarize the remarkable achievements in peroxisome engineering in yeast, the industrially popular biomanufacturing chassis host, to produce various biocompounds. We also review progress in plant peroxisome engineering, a field that has already exhibited high potential in both biomanufacturing and agriculture. Moreover, we outline various experimentally validated strategies to improve the efficiency of engineered pathways in peroxisomes, as well as prospects of peroxisome engineering. Subcellular compartmentalization of metabolic pathways plays a crucial role in metabolic engineering. The peroxisome has emerged as a highly valuable and promising compartment for organelle engineering, particularly in the fields of biological manufacturing and agriculture. In this review, we summarize the remarkable achievements in peroxisome engineering in yeast, the industrially popular biomanufacturing chassis host, to produce various biocompounds. We also review progress in plant peroxisome engineering, a field that has already exhibited high potential in both biomanufacturing and agriculture. Moreover, we outline various experimentally validated strategies to improve the efficiency of engineered pathways in peroxisomes, as well as prospects of peroxisome engineering.

• Publisher: Elsevier Ltd,Elsevier Limited
• Subject: Agriculture / Agriculture - methods / Alcohol / Biosynthesis / Biotechnology / Carbon / Chloroplasts / Efficiency / Engineering / Enzymes / Host plants / Internal Medicine / Metabolic engineering / Metabolic Engineering - methods / Metabolic Networks and Pathways - genetics / Metabolic pathways / Metabolism / Metabolites / Mitochondria / Natural products / Oxidation / peroxisome / peroxisomes / Peroxisomes - metabolism / plant / Plants - genetics / Plants - metabolism / Proteins / Saccharomyces cerevisiae - genetics / Saccharomyces cerevisiae - metabolism / subcellular compartmentalization / Sugarcane / synthetic biology / Yeast / yeasts