Journal Article

Transcription factor-associated combinatorial epigenetic pattern reveals higher transcriptional activity of TCF7L2-regulated intragenic enhancers

Li, Tianbao, Jin, Victor X., Bonneville, Russell, Liu, Qi

2017

Overview

Background Recent studies have suggested that combinations of multiple epigenetic modifications are essential for controlling gene expression. Despite numerous computational approaches have been developed to decipher the combinatorial epigenetic patterns or “epigenetic code”, none of them has explicitly addressed the relationship between a specific transcription factor (TF) and the patterns. Methods Here, we developed a novel computational method, T-cep, for annotating chromatin states associated with a specific TF. T-cep is composed of three key consecutive modules: (i) Data preprocessing, (ii) HMM training, and (iii) Potential TF-states calling. Results We evaluated T-cep on a TCF7L2-omics data. Unexpectedly, our method has uncovered a novel set of TCF7L2-regulated intragenic enhancers missed by other software tools, where the associated genes exert the highest gene expression. We further used siRNA knockdown, Co-transfection, RT-qPCR and Luciferase Reporter Assay not only to validate the accuracy and efficiency of prediction by T-cep, but also to confirm the functionality of TCF7L2-regulated enhancers in both MCF7 and PANC1 cells respectively. Conclusions Our study for the first time at a genome-wide scale reveals the enhanced transcriptional activity of cell-type-specific TCF7L2 intragenic enhancers in regulating gene expression.

• Publisher: BioMed Central,Springer Nature B.V,BMC
• Subject: Algorithms / Animal Genetics and Genomics / Biomedical and Life Sciences / Cancer / Chromatin / Combinatorial analysis / Computer applications / DNA methylation / Enhancer Elements, Genetic - genetics / Enhancers / Epigenesis, Genetic / Epigenetics / Gene expression / Genetic Loci - genetics / Genomes / Genomics / Human and rodent genomics / Humans / Hypotheses / Intragenic enhancer / Life Sciences / MCF-7 Cells / MCF7 / Methodology / Methodology Article / Microarrays / Microbial Genetics and Genomics / PANC1 / Plant Genetics and Genomics / Probability / Proteomics / siRNA / Social exclusion / Software / Software development tools / Studies / T-cep / TCF7L2 / Teaching methods / Transcription Factor 7-Like 2 Protein - metabolism / Transcription factors / Transcription, Genetic / Transfection