Journal Article

Genome Analysis of a Novel Clade b Betabaculovirus Isolated from the Legume Pest Matsumuraeses phaseoli (Lepidoptera: Tortricidae)

Miao, Lin, Meng, Qian, Zhang, Huan, Chen, Jun, Xu, Yuan, Shu, Ruihao, Jin, Wenyi, Cheng, Luqiang, Qin, Qilian, Liang, Hongbin

2020

Overview

Matsumuraeses phaseoli is a Lepidopteran pest that primarily feeds on numerous species of cultivated legumes, such as Glycine and Phaseolus. It is widely distributed in northeast Asia. A novel granulovirus, designated as Matsumuraeses phaseoli granulovirus (MaphGV), was isolated from pathogenic M. phaseoli larvae that dwell in rolled leaves of Astragalus membranaceus, a Chinese medicinal herb. In this study, using next-generation sequencing, we report the complete genome of MaphGV. MaphGV genome comprises a double-stranded DNA of 116,875 bp, with 37.18% GC content. It has 128 hypothetical open reading frames (ORFs). Among them, 38 are baculovirus core genes, 18 are lepidopteran baculovirus conserved genes, and 5 are unique to Baculoviridae. MaphGV has one baculovirus repeat ORF (bro) and three inhibitors of apoptosis proteins (iap), including a newfound iap-6. We found two atypical baculoviral homologous regions (hrs) and four direct repeats (drs) in the MaphGV genome. Based on phylogenetic analysis, MaphGV belongs to Clade b of Betabaculovirus and is closely related to Cydia pomonellagranulovirus (CpGV) and Cryptophlebia leucotretagranulovirus (CrleGV). This novel baculovirus discovery and sequencing are invaluable in understanding the evolution of baculovirus and MaphGV may be a potential biocontrol agent against the bean ravaging pest.

• Publisher: MDPI AG,MDPI
• Subject: Animals / Apoptosis / Asia / Astragalus membranaceus / Astragalus propinquus / baculovirus genome / Base Composition / beans / Betabaculovirus / Biological control / biological control agents / Cryptophlebia / Cydia / DNA / DNA, Viral - genetics / Genes / Genome, Viral / Genomes / granulovirus / Granulovirus - genetics / Granulovirus - isolation & purification / Herbal medicine / high-throughput nucleotide sequencing / IAP protein / Infections / insect pests / larvae / leaves / Legumes / Lepidoptera - virology / Maximum likelihood method / Medicinal plants / Morphology / Next-generation sequencing / Open reading frames / Pest control / Pest Control, Biological - methods / pest management / Phaseolus / Phylogenetics / Phylogeny / proteins / Scanning electron microscopy / Traditional Chinese medicine / Viruses